A sensitive detection assay based on signal amplification technology for Alzheimer's disease's early biomarker in exosome
| Autor: | Jie Zhou, Chong Sun, Shizhen Geng, Weiran Ye, Lingchang Meng, Qiaolei Wang |
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| Rok vydání: | 2018 |
| Předmět: |
Models
Molecular Dna duplex 02 engineering and technology Exosomes Biochemistry Exosome Protein Structure Secondary Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Alzheimer Disease Limit of Detection Environmental Chemistry Humans Spectroscopy Detection limit Amyloid beta-Peptides Chemistry Oxides Delayed treatment 021001 nanoscience & nanotechnology Peptide Fragments Biomarker Biophysics Graphite Protein Multimerization 0210 nano-technology Signal amplification 030217 neurology & neurosurgery DNA Biomarkers |
| Zdroj: | Analytica chimica acta. 1022 |
| ISSN: | 1873-4324 |
| Popis: | Alzheimer's disease (AD) considered as the third health "killer" has seriously threatened the health of the elderly. However, the modern diagnostic strategies of AD present several disadvantages: the low accuracy and specificity resulting in some false-negative diagnoses, and the poor sensitivity leading to a delayed treatment. In view of this situation, a enzyme-free and target-triggered signal amplification strategy, based on graphene oxide (GO) and entropy-driven strand displacement reaction (ESDR) principle, was proposed. In this strategy, when the hairpin structure probes (H)specially binds with beta-amyloid-(1-42) oligomers (Aβ42 oligomers), it's structure will be opened, causing the bases complementary to FAM-labeled replacement probes R (R1 and R2) exposed. At this time, R1 and R2 will hybridize with H, resulting in the bound Aβ42 oligomers released. The released Aβ42 oligomers would participate in the next cycle reaction, making the signal amplified. As a quencher, GO could absorb the free single-stranded DNA R1 and R2 and quench their fluorescence; however, the DNA duplex still exists free and keeps its signal-on. Through the detection of Aβ42 oligomers in exosomes, this ultrasensitive detection method with the advantages of low limit of detection (LOD, 20 pM), great accuracy, excellent precision and convenience provides an excellent prospect for AD's early diagnosis. |
| Databáze: | OpenAIRE |
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