Isolation and characterization of two novel, closely related ATF cDNA clones from HeLa cells
| Autor: | Mireille Gaire, Claude Kedinger, Bruno Chatton |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Leucine zipper
viruses genetic processes Molecular Sequence Data Biology DNA-binding protein environment and public health Epitopes Complementary DNA Sequence Homology Nucleic Acid Genetics Humans Amino Acid Sequence Cloning Molecular Cyclic AMP Response Element-Binding Protein Peptide sequence Transcription factor Base Sequence cDNA library fungi Nucleic acid sequence Promoter DNA Exons Molecular biology DNA-Binding Proteins HeLa Cells |
| Zdroj: | Nucleic acids research. 18(12) |
| ISSN: | 0305-1048 |
| Popis: | ATF or CRE binding proteins are cellular transcription factors involved in the regulation of adenovirus Ela-responsive and cellular cAMP-inducible promoters. We report the isolation from a HeLa cell cDNA library of two clones that encode proteins with specific ATF/CRE DNA binding activity. The two clones differ by a 63 bp element which is retained in one (ATF-a) and deleted from the other (ATF-a delta) and which may correspond to an alternative exon. The peptide sequences (483 and 462 amino acids, respectively) derived from each of these cDNAs are identical, except for the additional 21 amino acids in ATF-a, but clearly differ from the other ATF/CREB proteins reported. All of them, however, share a conserved leucine zipper domain also found in other transcription factors. ATF-a and ATF-a delta therefore represent two closely related members of a larger multigene family of proteins that interact with conserved promoter elements. |
| Databáze: | OpenAIRE |
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