Complexity and Diversity of the Mammalian Sialome Revealed by Nidovirus Virolectins
| Autor: | Langereis, Martijn A, Bakkers, Mark J G, Deng, Lingquan, Padler-Karavani, Vered, Vervoort, Stephin J, Hulswit, Ruben J G, van Vliet, Arno L W, Gerwig, Gerrit J, de Poot, Stefanie A H, Boot, Willemijn, van Ederen, Anne Marie, Heesters, Balthasar A, van der Loos, Chris M, van Kuppeveld, Frank J M, Yu, Hai, Huizinga, Eric G, Chen, Xi, Varki, Ajit, Kamerling, Johannis P, de Groot, Raoul J, Bio organic Chemistry, Crystal and Structural Chemistry, dI&I I&I-1, LS Virologie, Sub Membrane Biochemistry & Biophysics, Afd Chemical Biology and Drug Discovery, Sub Crystal and Structural Chemistry |
|---|---|
| Přispěvatelé: | Bio organic Chemistry, Crystal and Structural Chemistry, dI&I I&I-1, LS Virologie, Sub Membrane Biochemistry & Biophysics, Afd Chemical Biology and Drug Discovery, Sub Crystal and Structural Chemistry |
| Rok vydání: | 2015 |
| Předmět: |
Medical Physiology
Nidovirales Biochemistry Genome Viral Non-U.S. Gov't lcsh:QH301-705.5 Mammals Regulation of gene expression 0303 health sciences biology Research Support Non-U.S. Gov't 030302 biochemistry & molecular biology Acetylation Lipids 3. Good health Cell biology Hemagglutinins Sialome Glycan 1.1 Normal biological development and functioning Hemagglutinins Viral Research Support Article General Biochemistry Genetics and Molecular Biology N.I.H 03 medical and health sciences Species Specificity Research Support N.I.H. Extramural Underpinning research Journal Article Animals Humans Differential expression 030304 developmental biology Biochemistry Genetics and Molecular Biology(all) Cell growth Proteins Extramural N-Acetylneuraminic Acid lcsh:Biology (General) Gene Expression Regulation Sialic Acids biology.protein Acetylesterase Generic health relevance Biochemistry and Cell Biology Viral Fusion Proteins Function (biology) Genetics and Molecular Biology(all) |
| Zdroj: | Cell Reports Cell Reports [E], 11(12), 1966. Cell Press Cell reports, vol 11, iss 12 Cell reports Cell Reports [E], 11(12), 1966. Elsevier Saunders Cell Reports, Vol 11, Iss 12, Pp 1966-1978 (2015) |
| ISSN: | 2211-1247 |
| Popis: | SUMMARY Sialic acids (Sias), 9-carbon-backbone sugars, are among the most complex and versatile molecules of life. As terminal residues of glycans on proteins and lipids, Sias are key elements of glycotopes of both cellular and microbial lectins and thus act as important molecular tags in cell recognition and signaling events. Their functions in such interactions can be regulated by post-synthetic modifications, the most common of which is differential Sia-O-acetylation (O-Ac-Sias). The biology of O-Ac-Sias remains mostly unexplored, largely because of limitations associated with their specific in situ detection. Here, we show that dual-function hemagglutinin-esterase envelope proteins of nidoviruses distinguish between a variety of closely related O-Ac-Sias. By using soluble forms of hemagglutinin-esterases as lectins and sialate-O-acetylesterases, we demonstrate differential expression of distinct O-Ac-sialoglycan populations in an organ-, tissue- and cell-specific fashion. Our findings indicate that programmed Sia-O-acetylation/de-O-acetylation may be critical to key aspects of cell development, homeostasis, and/or function. Graphical Abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|