Alkaline extractability of pectic arabinan and galactan and their mobility in sugar beet and potato cell walls
| Autor: | Corinne Rondeau-Mouro, Marie-Christine Ralet, Jean-François Thibault, Agata Zykwinska, Catherine Garnier |
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| Přispěvatelé: | Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
0106 biological sciences
food.ingredient POLYSACCHARIDES PARIETAUX Polymers and Plastics Pectin XYLOGLUCAN 02 engineering and technology GALACTAN Polysaccharide 01 natural sciences Cell wall chemistry.chemical_compound food PECTIN Materials Chemistry SPECTROSCOPIE RMN DU SOLIDE chemistry.chemical_classification Chromatography biology EXPERIMENTAL DESIGN Organic Chemistry Extraction (chemistry) ARABINAN food and beverages Galactan 021001 nanoscience & nanotechnology biology.organism_classification SOLID STATE 13C NMR SPECTROSCOPY Xyloglucan [CHIM.POLY]Chemical Sciences/Polymers chemistry Biochemistry Composition (visual arts) Sugar beet 0210 nano-technology 010606 plant biology & botany |
| Zdroj: | Carbohydrate Polymers Carbohydrate Polymers, Elsevier, 2006, 65, pp.510-520. ⟨10.1016/j.carbpol.2006.02.012⟩ |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2006.02.012⟩ |
| Popis: | International audience; The organisation of sugar beet and potato cell walls was studied using alkaline extractions following a response surface methodology, simultaneously with solid-state C-13 NMR spectroscopy. The influence of two extraction parameters: NaOH concentration (0.05, 0.275, 0.5 M) and temperature (40, 65, 90 degrees C) on the composition (neutral and acidic sugars) of the residues recovered was established. Treatments of increasing harshness progressively washed off non-cellulosic polysaccharides from the cell walls. Alkaline treatments applied to sugar beet cell wall material (SB-CWM) revealed the presence of diverse pectin populations. The existence of distinct pectin populations in potato cell wall material (P-CWM) was less outstanding. Solid-state C-13 NMR applied to SB-CWM and P-CWM and residues after treatment by 0.275 M NaOH at 65 degrees C revealed two fractions of pectic arabinan and galactan side chains. One fraction was highly mobile, whereas the other one displayed restricted mobility. |
| Databáze: | OpenAIRE |
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