Cross-reactivity of human molecular markers for detection of prethrombotic states in various animal species
| Autor: | C. Legrand, Jean-Pierre Cazenave, Catherine Ravanat, Ludovic Drouet, J P Maffrand, F. Incardona, M. Freund, B. Boneu, Jean-Marc Herbert, Y. Cadroy, F. Dol, Jacqueline Roussi |
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| Rok vydání: | 1995 |
| Předmět: |
Swine
Antithrombin III Guinea Pigs Zoology Platelet Membrane Glycoproteins Biology medicine.disease_cause Cross-reactivity Thromboplastin Mice Dogs Animal model Species Specificity biology.animal medicine Animals Humans Fibrinopeptide Prothrombin fragment Rats Wistar Animal species Blood Coagulation Fibrinolysis Thrombosis Hematology General Medicine Platelet Activation Peptide Fragments Rats Biochemistry Female Prothrombin Rabbits Chickens Biomarkers Papio Peptide Hydrolases circulatory and respiratory physiology Baboon |
| Zdroj: | Blood Coagulation & Fibrinolysis. 6:446-455 |
| ISSN: | 0957-5235 |
| DOI: | 10.1097/00001721-199507000-00012 |
| Popis: | The aim of the present study was to investigate the reactivity of immunoreagents developed for clinical applications in humans in different animal species (hen, mouse, rat, rabbit, guinea-pig, dog, pig, sheep, baboon). Prothrombin fragment 1 + 2, thrombin-antithrombin III complex and fibrinopeptide A were tested for coagulation, platelet factor 4 and beta-thromboglobulin for platelet activation, glycoprotein IIb-IIIa, glycoprotein Ib and P-selectin for platelet membrane glycoproteins, D-dimers for fibrinolysis, thrombomodulin for activation of endothelial cells and thrombospondin and von Willebrand factor for adhesive proteins. Prothrombin fragment 1 + 2, platelet factor 4, beta-thromboglobulin and D-dimers were revealed only in baboons. Fibrinopeptide A was well detected in baboons but weakly in mice, dogs, pigs and sheep. Whereas glycoprotein IIb-IIIa was revealed on guinea-pig, dog and sheep platelets and glycoprotein Ib on rabbit and dog platelets, P-selectin and thrombomodulin were never detected. Thrombospondin was revealed in hens, mice, rats, guinea-pigs, pigs, sheep and baboons and von Willebrand factor in mice, rats, guinea-pigs, dogs, pigs, sheep and baboons. Interestingly, thrombin-antithrombin III complex (TAT) was detected in all species tested except the hen. A time- and dose-dependent increase in TAT was observed when rats, dogs or pigs were infused with thromboplastin (4.5-450 microliters/kg/h), while administration of hirudin (1 mg/kg) abolished this TAT generation. Thus, the TAT immunoassay could provide a tool for the screening of antithrombotic drugs in a number of animal species. However, the possibility of using a wider panel of human immunoreagents would appear to be restricted to baboons which display good species cross-reactivity. |
| Databáze: | OpenAIRE |
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