Design and performance of a dark-field probe with confocal Raman spectroscopy for ophthalmic applications
| Autor: | Franciscus H. M. Jongsma, Roel J. Erckens, Carroll A.B. Webers, Shuo Zhang, Tos T. J. M. Berendschot |
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| Přispěvatelé: | Medical Image Analysis, Oogheelkunde, RS: MHeNs - R3 - Neuroscience, MUMC+: MA UECM Oogartsen ZL (9), MUMC+: MA UECM Oogartsen MUMC (9), MUMC+: *MA Oogheelkunde (3), MUMC+: University Eye Center Maastricht (3), RS: NUTRIM - R1 - Obesity, diabetes and cardiovascular health |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Materials science
dark‐ Confocal Analytical chemistry Aqueous humor Dark field microscopy field illumination symbols.namesake Raman spectroscopy symbols Concentration curve ocular drug General Materials Science Phenylephrine hcl Ray tracing (graphics) aqueous humor dark-field illumination Confocal raman spectroscopy Spectroscopy |
| Zdroj: | Journal of Raman Spectroscopy, 52(7), 1371-1375. Wiley Journal of Raman Spectroscopy, 52(7), 1371-1375. John Wiley & Sons Inc. |
| ISSN: | 0377-0486 |
| DOI: | 10.1002/jrs.6125 |
| Popis: | A dark-field ophthalmic contact probe for analysis of aqueous humor (AH) was designed by ray tracing and constructed in quartz glass for experimental use as a prototype probe in a confocal Raman spectroscopic setup. The misalignment tolerance of this probe was theoretically evaluated, whereas the optical performance was experimentally evaluated by a concentration curve of the ocular drug phenylephrine HCl in an artificial eye model. The results indicated a ninefold improvement in misalignment tolerance and a 36-fold improvement in the signal-to-noise ratio at the phenylephrine HCl 1002 cm(-1) peak compared with a previous design. The prototype showed a good linear fit with the phenylephrine HCl concentration, with an R-2 of 0.996. Phenylephrine HCl concentration down to 0.1% is detectable. |
| Databáze: | OpenAIRE |
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