CMF608—a novel mechanical strain-induced bone-specific protein expressed in early osteochondroprogenitor cells
| Autor: | Alexander Faerman, Elena Feinstein, Anat Moshayov, Orit Segev, Helen Gottlieb, Itzhak Binderman, Eugeniy Kazanov, Dror Robinson, Paz Einat, Merav Beiman, Zvi Nevo, Shlomit Boguslavsky, Hagar Kalinski, Rami Skaliter, Aviva Samach, Hagit Turam, Anna Gelfand |
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| Rok vydání: | 2004 |
| Předmět: |
Cell type
Histology Physiology Endocrinology Diabetes and Metabolism Blotting Western Molecular Sequence Data Biology Osteocytes Bone and Bones Fractures Bone Chondrocytes Complementary DNA medicine Animals Humans Amino Acid Sequence Endochondral ossification Cells Cultured In Situ Hybridization Oligonucleotide Array Sequence Analysis Endosteum Periosteum Base Sequence Sequence Homology Amino Acid Reverse Transcriptase Polymerase Chain Reaction Stem Cells Skull Mesenchymal stem cell Transfection Blotting Northern Up-Regulation Cell biology medicine.anatomical_structure Biochemistry Protein Biosynthesis Ectopic expression Stress Mechanical |
| Zdroj: | Bone. 34:246-260 |
| ISSN: | 8756-3282 |
| Popis: | Microarray gene expression analysis was utilized to identify genes upregulated in primary rat calvaria cultures in response to mechanical force. One of the identified genes designated CMF608 appeared to be novel. The corresponding full-length cDNA was cloned and characterized in more details. It encodes a putative 2597 amino acid protein containing N-terminal signal peptide, six leucine-rich repeats (LRRs), and 12 immunoglobulin-like repeats, 10 of which are clustered within the C-terminus. Expression of CMF608 is bone-specific and the main type of CMF608-positive cells is mesenchymal osteochondroprogenitors with fibroblast-like morphology. These cells reside in the perichondral fibrous ring of La Croix, periosteum, endosteum of normal bone as well as in the activated periosteum and early fibrous callus generated postfracture. Expression of CMF608 is notably absent from the regions of endochondral ossification. Mature bone cell types do not produce CMF608 with the exception of chondrocytes of the tangential layer of the articular cartilage, which are thought to be under constant mechanical loading. Ectopic expression of CMF608 in HEK293T cells shows that the protein is subjected to post-translational processing and its N-terminal approximately 90 kDa polypeptide can be found in the conditioned medium. Ectopic expression of either the full-length cDNA of CMF608 or of its N-terminal region in CMF608-negative ROS17/2.8 rat osteosarcoma cells results in transfected clones displaying increased proliferation rate and the characteristics of less-differentiated osteoblasts compared to the control cells. Our data indicate that CMF608 is a unique marker of early osteochondroprogenitor cells. We propose that it could be functionally involved in maintenance of the osteochondroprogenitor cells pool and its down-regulation precedes terminal differentiation. |
| Databáze: | OpenAIRE |
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