Prevention by the Natural Artocarpin of Morphological and Biochemical Alterations on UVB-Induced HaCaT Cells
| Autor: | Francois Grandmottet, Céline Viennet, Kunlathida Luangpraditkun, Marion Tissot, Jarupa Viyoch, Pensri Charoensit, Anupong Joompang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Keratinocytes Aging Programmed cell death Antioxidant Article Subject Ultraviolet Rays medicine.medical_treatment Cell Radiation-Protective Agents Biochemistry Antioxidants 03 medical and health sciences 0302 clinical medicine medicine HaCaT Cells Humans Secretion Fragmentation (cell biology) QH573-671 Chemistry Caspase 3 Cell Biology General Medicine Fas receptor Apoptotic body Cell biology HaCaT 030104 developmental biology medicine.anatomical_structure Mannose-Binding Lectins 030220 oncology & carcinogenesis Plant Lectins Reactive Oxygen Species Cytology Artocarpus Research Article |
| Zdroj: | Oxidative Medicine and Cellular Longevity, Vol 2021 (2021) Oxidative Medicine and Cellular Longevity |
| ISSN: | 1942-0900 |
| DOI: | 10.1155/2021/5067957 |
| Popis: | Natural substances have gained considerable attention for skin protection against UV light reactions. Artocarpus altilis plant’s heartwood extract is comprised of artocarpin as a major substance, already known for its interesting biological attributes as an antimicrobial, an anti-inflammatory, an antioxidant, and a melanogenesis inhibitor. The present work clarified the mechanism of natural artocarpin (NAR) with a purity of approximately 99% against the effects of UVB-induced HaCaT keratinocyte apoptosis. The indicated results showed that NAR suppresses free radical production (ROS and nitrite) and apoptosis-related molecule activation (caspase-3, p-p53, p-p38, and NF-κB p65) and secretion (TNF-α). Additionally, NAR prevented structural damages (nuclei condensation and fragmentation, apoptotic body formation, impaired cell adherence and round cell shape, disruption of F-actin filament, and clustering of cell death receptor CD95/Fas) and biophysical changes (plasma membrane rigidification). Thus, NAR acts directly from scavenging free radicals generated by UV and indirectly by suppressing morphological and biochemical UV-induced cell damages. Its biological effects are mainly attributed to antioxidant and antiapoptotic properties. Taken together, NAR could be considered as an effective natural product for photoprotective formulations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text