Influence of chronic azithromycin treatment on the composition of the oropharyngeal microbial community in patients with severe asthma
| Autor: | Guy Joos, Mario Vaneechoutte, Paul Jordens, Guy Brusselle, Ellen Deschepper, Guido Lopes dos Santos Santiago, Pieter Deschaght, Chris Verhofstede, Dries Vaneechoutte, Kenny Dauwe |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Adult
DNA Bacterial Male 0301 basic medicine Microbiology (medical) DIVERSITY lcsh:QR1-502 Oropharynx Azithromycin Microbiology lcsh:Microbiology Prevotella melaninogenica Young Adult 03 medical and health sciences RNA Ribosomal 16S Streptococcus mitis Medicine and Health Sciences Prevotella medicine Humans Microbiome Phylogeny Bacteria biology Microbiota Biology and Life Sciences High-Throughput Nucleotide Sequencing Fusobacteria Biodiversity Middle Aged Oral microbiome biology.organism_classification Antibiotic treatment Asthma Oropharyngeal microbiome 030104 developmental biology Genes Bacterial Female Oral Microbiome Fusobacterium nucleatum Sequence Analysis Research Article medicine.drug |
| Zdroj: | BMC Microbiology, Vol 17, Iss 1, Pp 1-12 (2017) BMC Microbiology BMC MICROBIOLOGY |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-017-1022-6 |
| Popis: | Background This study of the oropharyngeal microbiome complements the previously published AZIthromycin in Severe ASThma (AZISAST) clinical trial, where the use of azithromycin was assessed in subjects with exacerbation-prone severe asthma. Here, we determined the composition of the oropharyngeal microbial community by means of deep sequencing of the amplified 16S rRNA gene in oropharyngeal swabs from patients with exacerbation-prone severe asthma, at baseline and during and after 6 months treatment with azithromycin or placebo. Results A total of 1429 OTUs were observed, of which only 59 were represented by more than 0.02% of the reads. Firmicutes, Bacteroidetes, Fusobacteria, Proteobacteria and Actinobacteria were the most abundant phyla and Streptococcus and Prevotella were the most abundant genera in all the samples. Thirteen species only accounted for two thirds of the reads and two species only, i.e. Prevotella melaninogenica and Streptococcus mitis/pneumoniae, accounted for one fourth of the reads. We found that the overall composition of the oropharyngeal microbiome in patients with severe asthma is comparable to that of the healthy population, confirming the results of previous studies. Long term treatment (6 months) with azithromycin increased the species Streptococcus salivarius approximately 5-fold and decreased the species Leptotrichia wadei approximately 5-fold. This was confirmed by Boruta feature selection, which also indicated a significant decrease of L. buccalis/L. hofstadtii and of Fusobacterium nucleatum. Four of the 8 treated patients regained their initial microbial composition within one month after cessation of treatment. Conclusions Despite large diversity of the oropharyngeal microbiome, only a few species predominate. We confirm the absence of significant differences between the oropharyngeal microbiomes of people with and without severe asthma. Possibly, long term azithromycin treatment may have long term effects on the composition of the oropharygeal microbiome in half of the patients. Electronic supplementary material The online version of this article (doi:10.1186/s12866-017-1022-6) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|