Pannier is a Transcriptional Target and Partner of Tinman during Drosophila Cardiogenesis
| Autor: | Kathleen M Gajewski, Anh Dang, Qian Zhang, Robert A. Schulz, Nancy Fossett, Young Ho Kim, Yongsok Kim, Cheol Yong Choi |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Mesoderm
Transcription Genetic Genes Insect D-MEF2 heart Biology protein interactions Protein–protein interaction 03 medical and health sciences 0302 clinical medicine Gene expression medicine Animals Drosophila Proteins Heart formation Molecular Biology Gene Cells Cultured DNA Primers 030304 developmental biology Genetics 0303 health sciences Base Sequence cardiogenic factors Tinman Genes Homeobox Drosophila embryogenesis Cell Biology Protein Structure Tertiary transcriptional enhancer Cell biology Repressor Proteins Pannier Enhancer Elements Genetic medicine.anatomical_structure Mutation embryonic structures Trans-Activators Homeobox Drosophila 030217 neurology & neurosurgery Function (biology) Transcription Factors Developmental Biology |
| Zdroj: | Developmental Biology. 233:425-436 |
| ISSN: | 0012-1606 |
| DOI: | 10.1006/dbio.2001.0220 |
| Popis: | During Drosophila embryogenesis, the homeobox gene tinman is expressed in the dorsal mesoderm where it functions in the specification of precursor cells of the heart, visceral, and dorsal body wall muscles. The GATA factor gene pannier is similarly expressed in the dorsal-most part of the mesoderm where it is required for the formation of the cardial cell lineage. Despite these overlapping expression and functional properties, potential genetic and molecular interactions between the two genes remain largely unexplored. Here, we show that pannier is a direct transcriptional target of Tinman in the heart-forming region. The resulting coexpression of the two factors allows them to function combinatorially in the regulation of cardiac gene expression, and a physical interaction of the proteins has been demonstrated in cultured cells. We also define functional domains of Tinman and Pannier that are required for their synergistic activation of the D-mef2 differentiation gene in vivo. Together, these results provide important insights into the genetic mechanisms controlling heart formation in the Drosophila model system. |
| Databáze: | OpenAIRE |
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