Sex Chromosome Analysis of Postmortem Corneal Endothelium After Sex-Mismatch Descemet Membrane Endothelial Keratoplasty
Autor: | Hein F. Sleddens, Silke Oellerich, Itay Lavy, Gerrit R. J. Melles, Marieke Bruinsma, Robert M. Verdijk, Perry S. Binder |
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Přispěvatelé: | Pathology |
Rok vydání: | 2017 |
Předmět: |
Male
medicine.medical_specialty Corneal endothelium Endothelium Cell Survival Fuchs Endothelial Dystrophy 03 medical and health sciences Sex Factors 0302 clinical medicine Ophthalmology Cornea medicine Humans Descemet Membrane In Situ Hybridization Fluorescence Aged Aged 80 and over Sex Chromosomes medicine.diagnostic_test business.industry Endothelium Corneal Fuchs' Endothelial Dystrophy Endothelial Cells Contact inhibition Cell migration Middle Aged Tissue Donors medicine.anatomical_structure 030221 ophthalmology & optometry Female business Wound healing Descemet Stripping Endothelial Keratoplasty 030217 neurology & neurosurgery Fluorescence in situ hybridization |
Zdroj: | Cornea, 36(1), 11-16. Lippincott Williams & Wilkins |
ISSN: | 0277-3740 |
Popis: | Purpose: To identify the origin of corneal endothelial cells (host or donor) present on grafts at various time points after Descemet membrane endothelial keratoplasty (DMEK), using fluorescence in situ hybridization (FISH) of sex chromosomes on post mortem corneas with sex mismatch between the donor and host. Methods: Corneoscleral buttons of 6 post mortem DMEK eyes of 4 patients, operated for Fuchs endothelial dystrophy, with an average postoperative time of 2.6 (±1.8) years (range, 7 months-4.5 years), of 2.5 (±1.7) years (range, 7 months-4 years), were processed for FISH detection of XX (female) or XY (male)-labeling signals in corneal endothelial cells in the central area of the DMEK graft. Two male patients underwent bilateral DMEK with grafts from female donors, and 2 female patients underwent unilateral DMEK and received a graft from a male donor. Results: FISH consistently showed the presence of donor endothelial cells across the graft area, with signaling of sex chromosomes opposite to the sex of the host. Conclusions: Donor endothelial cells may survive up to 4.5 years after DMEK. If so, the lower incidence of allograft rejection in DMEK may not be explained by early host cell replacement. Potential host cell migration may be limited by donor/recipient cell-cell contact inhibition. |
Databáze: | OpenAIRE |
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