Tripsazea, a Novel Trihybrid of Zea mays, Tripsacum dactyloides, and Zea perennis
| Autor: | Tingzhao Rong, Li Yang, Yang Chunyan, He Jianmei, Mingjun Cheng, Ebenezer Kofi Sam, Wen Xiaodong, Yan Xu, He Ruyu, Li Huaxiong, Ping Zhang, Zhao Yanli, Wu Zizhou, Li Xiaofeng, Qilin Tang, Li Yingzheng |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Perennial plant forage breeding Vegetative reproduction Karyotype trihybrid Investigations QH426-470 Poaceae 01 natural sciences Genome Chromosomes Plant DNA sequencing Polyploidy zea mays 03 medical and health sciences maize improvement Botany Tripsacum dactyloides Genetics zea perennis Molecular Biology Tripsacum Crosses Genetic Genetics (clinical) 030304 developmental biology 0303 health sciences biology Reproduction Chromosome biology.organism_classification tripsacum dactyloides Plant Breeding Phenotype Zea perennis Hybridization Genetic Genome Plant 010606 plant biology & botany |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 10, Iss 2, Pp 839-848 (2020) G3: Genes|Genomes|Genetics |
| ISSN: | 2160-1836 |
| DOI: | 10.1534/g3.119.400942 |
| Popis: | A trispecific hybrid, MTP (hereafter called tripsazea), was developed from intergeneric crosses involving tetraploid Zea mays (2n = 4x = 40, genome: MMMM), tetraploid Tripsacum dactyloides (2n = 4x = 72, TTTT), and tetraploid Z. perennis (2n = 4x = 40, PPPP). On crossing maize-Tripsacum (2n = 4x = 56, MMTT) with Z. perennis, 37 progenies with varying chromosome numbers (36-74) were obtained, and a special one (i.e., tripsazea) possessing 2n = 74 chromosomes was generated. Tripsazea is perennial and expresses phenotypic characteristics affected by its progenitor parent. Flow cytometry analysis of tripsazea and its parents showed that tripsazea underwent DNA sequence elimination during allohexaploidization. Of all the chromosomes in diakinesis I, 18.42% participated in heterogenetic pairing, including 16.43% between the M- and P-genomes, 1.59% between the M- and T-genomes, and 0.39% in T- and P-genome pairing. Tripsazea is male sterile and partly female fertile. In comparison with previously synthesized trihybrids containing maize, Tripsacum and teosinte, tripsazea has a higher chromosome number, higher seed setting rate, and vegetative propagation ability of stand and stem. However, few trihybrids possess these valuable traits at the same time. The potential of tripsazea is discussed with respect to the deployment of the genetic bridge for maize improvement and forage breeding. |
| Databáze: | OpenAIRE |
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