Host Cell Polarity Proteins Participate in Innate Immunity to Pseudomonas aeruginosa Infection
| Autor: | Travis R. Ruch, David M. Bryant, Arlinet Kierbel, Ross J. Metzger, Anirban Datta, Keith E. Mostov, Torsten Wittmann, Cindy S. Tran, Joanne N. Engel, Yoni Eran, Paul Brakeman |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
rac1 GTP-Binding Protein
Cancer Research Cystic Fibrosis Phosphatidylinositol 3-Kinases Innate 2.2 Factors relating to the physical environment polarity Aetiology Lung Epithelial polarity NF-kappa B Cell Polarity Cell biology Infectious Diseases medicine.anatomical_structure Medical Microbiology Pseudomonas aeruginosa Pneumonia & Influenza Infection CIENCIAS NATURALES Y EXACTAS Otras Ciencias Biológicas Immunology Nerve Tissue Proteins RAC1 Flagellum Biology Microbiology Article Cell Line Vaccine Related Ciencias Biológicas Rare Diseases Immune system Biodefense Immunology and Microbiology(all) Virology medicine Humans Pseudomonas Infections Secretion Molecular Biology Polarity (international relations) Innate immune system Prevention Immunity epithelia Epithelial Cells Pneumonia Immunity Innate Epithelium Emerging Infectious Diseases Parasitology native immunity Carrier Proteins |
| Zdroj: | Cell host & microbe, vol 15, iss 5 |
| Popis: | The mucosal epithelium consists of polarized cells with distinct apical and basolateral membranes that serve as functional and physical barriers to external pathogens. The apical surface of the epithelium constitutes the first point of contact between mucosal pathogens, such as Pseudomonas aeruginosa, and their host. We observed that binding of P. aeruginosa aggregates to the apical surface of polarized cells led to the striking formation of an actin-rich membrane protrusion with inverted polarity, containing basolateral lipids and membrane components. Such protrusions were associated with a spatially localized host immune response to P. aeruginosa aggregates that required bacterial flagella and a type III secretion system apparatus. Host protrusions formed de novo underneath bacterial aggregates and involved the apical recruitment of a Par3/Par6α/aPKC/Rac1 signaling module for a robust, spatially localized host NF-κB response. Our data reveal a role for spatiotemporal epithelial polarity changes in the activation of innate immune responses. Fil: Tran, Cindy S.. University of California; Estados Unidos Fil: Eran, Yonatan. University of California; Estados Unidos Fil: Ruch, Travis. University of California; Estados Unidos Fil: Bryant, David. University of California; Estados Unidos Fil: Datta, Aniriban. University of California; Estados Unidos Fil: Brakeman, Paul. University of California; Estados Unidos Fil: Kierbel, Arlinet Verónica. Instituto Pasteur de Montevideo; Uruguay. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina Fil: Wittman, Torsten. University of California; Estados Unidos Fil: Metzger, Ross. University of California; Estados Unidos Fil: Mostov, Keith. University of California; Estados Unidos Fil: Engel, Joanne. University of California; Estados Unidos |
| Databáze: | OpenAIRE |
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