miR‑454 promotes survival and induces oxaliplatin resistance in gastric carcinoma cells by targeting CYLD
| Autor: | Chao-Qun Huang, Xiao-Jun Yang, Jiuyang Liu, Xuekai Pan, Bin Xiong, Maohui Feng, Chun-Wei Peng |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cancer Research Cell Biology miR-454 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) medicine Gene silencing Oncogene oxaliplatin cylindromatosis Cancer Articles General Medicine Transfection Cell cycle medicine.disease Oxaliplatin 030104 developmental biology medicine.anatomical_structure SGC-7901 cells 030220 oncology & carcinogenesis Cancer cell Cancer research medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2020.8655 |
| Popis: | MicroRNA-454 (miR-454), is involved in the progression of various types of cancers. The present study aimed to evaluate the effect of miR-454 on the progression of gastric cancer. SGC-7901 cells overexpressing or silencing miR454 were constructed via transfection and the survival rate of the cells was determined. The relationship between miR-454 and cylindromatosis (CYLD) was explored and the influence of miR-454 on oxaliplatin resistance was investigated in SGC-7901 cells. It was determined that overexpression of miR-454 increased the number of colonies and reduced apoptosis rate of SGC-7901 cells. The CYLD gene was identified as a direct target of miR-454. miR-454 overexpression downregulated the expression of CYLD, leading to an increase in SGC-7901 cell proliferation. Finally, miR-454 was also demonstrated to induce resistance to oxaliplatin in gastric cancer cells. In conclusion, the present in vitro findings suggested that miR-454 might be a novel therapeutic target for gastric cancer. |
| Databáze: | OpenAIRE |
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