β-Carotene and its physiological metabolites: Effects on oxidative status regulation and genotoxicity in in vitro models
| Autor: | Daniela Ribeiro, Marta Nunes da Silva, Ana T. Rufino, Adelaide Sousa, Félix Carvalho, José Miguel P. Ferreira de Oliveira, Pedro Nicola, Eduarda Fernandes, Marisa Freitas |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
medicine.medical_treatment Oxidative phosphorylation In Vitro Techniques Toxicology medicine.disease_cause Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0404 agricultural biotechnology medicine Humans Hydrogen peroxide Carotenoid 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chemistry Mutagenicity Tests Carotene 04 agricultural and veterinary sciences General Medicine beta Carotene 040401 food science Oxidative Stress Biochemistry Peroxynitrite Genotoxicity Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 141 |
| ISSN: | 1873-6351 |
| Popis: | Carotenoids are ubiquitously distributed in nature, β-carotene being the most frequently found carotenoid in the human diet. In the human body, β-carotene is absorbed, distributed and metabolized by enzymatic and/or non-enzymatic oxidant cleavage into several metabolites. Despite the broadly accepted biological value of β-carotene, it has also been considered a double-edged sword, mainly due to its potential antioxidant versus pro-oxidant behaviour. In this sense, the aim of this work was to scrutinize the antioxidant or pro-oxidant potential of β-carotene and its metabolites, namely trans-β-apo-8'-carotenal and β-ionone. Several parameters were evaluated in this study, viz. their effects on reactive species production, both in human whole blood and neutrophils; their effects on lipid peroxidation, in the absence and presence of peroxynitrite anion (ONOO-) or hydrogen peroxide (H2O2), using a synaptosomal model; and finally, their putative genotoxic effects in the human hepatic HepG2 cell line. In general, depending on the cellular model and conditions tested, β-carotene and its metabolites revealed antioxidant effects to varying degrees without significant pro-oxidant or genotoxic effects. |
| Databáze: | OpenAIRE |
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