Mite-antigen Stimulates MAL Expression in Peripheral Blood T Cells of Mite-sensitive Subjects
| Autor: | Yuji Sugita, Tadahilo Oshida, Akira Akasawa, Naoko Nagata, Yoh-ichi Seki, Gozoh Tsujimoto, Hirohisa Saito, Tohru Arai, Masato Kubo, Ning Lu Yoshida |
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| Jazyk: | angličtina |
| Předmět: |
endocrine system diseases
biology atopic dermatitis T cell CD3 glycolipid-enriched membrane microdomains General Medicine MAL Peripheral blood mononuclear cell Molecular biology lipid raft Interleukin 21 medicine.anatomical_structure Immune system Downregulation and upregulation Immunology Gene expression parasitic diseases medicine biology.protein Immunology and Allergy Secretion lipids (amino acids peptides and proteins) |
| Zdroj: | Allergology International. (2):273-282 |
| ISSN: | 1323-8930 |
| DOI: | 10.2332/allergolint.54.273 |
| Popis: | Background Differential gene expression in CD3+ T cells from allergic patients stimulated with allergen will provide important information on the responses of T cells. Methods After stimulation of peripheral blood mononuclear cells (PBMCs) with mite extracts, levels of gene transcription were examined in CD3+ T cells from allergic patients. Results Stimulation of PBMCs from mite specific IgE positive subjects resulted in specific upregulation of MAL transcription levels that was mediated by IL-4 secretion. The MAL protein in IL-4 stimulated primary T cells preferentially localized in glycolipid-enriched membrane (GEM) microdomains. When MAL was exogenously expressed in primary T cells, CD3ζ was concomitantly enriched, along with the expression of MAL, in GEM microdomains. Conclusions GEMs are important for the formation and stabilization of TCR signaling complexes. Therefore, MAL may play a role in the formation of GEMs in activated T cells and the high expression of MAL may contribute to Th2 immune response. |
| Databáze: | OpenAIRE |
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