Dose dependent effect of C-type natriuretic peptide signaling in glycosaminoglycan synthesis during TGF-β1 induced chondrogenic differentiation of mesenchymal stem cells

Autor: Sema Serter, Berna Tezcan, Esat Kiter, A. Cevik Tufan
Rok vydání: 2010
Předmět:
NPR-B
cell migration
Physiology
transforming growth factor beta1
Cell Separation
Glycosaminoglycan
transcription factor Sox9
Natriuretic Peptide
Brain
mesenchymal stem cell
Glycosaminoglycans
Reverse Transcriptase Polymerase Chain Reaction
adult
article
Cell Differentiation
General Medicine
protein function
Middle Aged
Immunohistochemistry
natriuretic peptide receptor B
cell assay
Cell biology
aged
priority journal
bone development
Adipogenesis
trabecular bone
natriuretic peptide type C
Antigens
Surface
CNP
aggrecan
Signal transduction
membrane antigen
Chondrogenesis
signal transduction
Signal Transduction
TGF-β
medicine.medical_specialty
in vitro study
Histology
Biology
Bone and Bones
adipogenesis
reverse transcription polymerase chain reaction
Transforming Growth Factor beta1
collagen type 2
Internal medicine
medicine
Humans
controlled study
human
alcian blue
protein expression
Aggrecan
Aged
human cell
Mesenchymal stem cell
nucleotide sequence
Mesenchymal Stem Cells
Natriuretic Peptide
C-Type
Cell Biology
human tissue
In vitro
glycosaminoglycan metabolism
cell membrane potential
Endocrinology
concentration response
protein analysis
GAG synthesis
Transforming growth factor
Zdroj: Journal of Molecular Histology. 41:247-258
ISSN: 1567-2387
1567-2379
DOI: 10.1007/s10735-010-9284-4
Popis: Recent investigations credited important roles to C-type natriuretic peptide (CNP) signaling during chondrogenesis. This study investigated the putative role of CNP in transforming growth factor (TGF)-β1 induced in vitro chondrogenic differentiation of mesenchymal stem cells (MSCs) in pellet culture. MSCs were derived from human trabecular bone and were characterized on the basis of their cell surface antigens and adipogenic, osteogenic, and chondrogenic differentiation potential. TGF-β1 induced chondrogenic differentiation and glycosaminoglycan (GAG) synthesis was analyzed on the basis of basic histology, collagen type II, Sox 9 and aggrecan expressions, and Alcian blue staining. Results revealed that human trabecular bone-derived MSCs express CNP and NPR-B analyzed on the basis of RT-PCR and immunohistochemistry. In pellet cultures of MSCs TGF-β1 successfully induced chondrogenic differentiation and GAG synthesis. RT-PCR analyses of both CNP and NPR-B during this process revealed an activation of this signaling pathway in response to TGF-β1. Similar cultures induced with TGF-β1 and treated with different doses of CNP showed that CNP supplementation at 10-8 and 10-7 M concentrations significantly increased GAG synthesis in a dose dependent manner, whereas at 10-6 M concentration this stimulatory effect was diminished. In conclusion, CNP/NPR-B signaling pathway is activated during TGF-β1 induced chondrogenic differentiation of human trabecular bone-derived MSCs and may strongly be involved in GAG synthesis during this process. This effect is likely to be a dose-dependent effect. © 2010 Springer Science+Business Media B.V.
Databáze: OpenAIRE
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