Arabidopsis TCP Transcription Factors Interact with the SUMO Conjugating Machinery in Nuclear Foci
| Autor: | André Djajasaputra, Georgios Vlachakis, Bas Beerens, Walter Gassmann, Harrold A. van den Burg, Benjamin J. Spears, Magdalena J Mazur, Michelle van der Gragt |
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| Přispěvatelé: | Molecular Plant Pathology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
yeast two-hybrid Two-hybrid screening genetic processes SUMO protein Laboratory of Virology Plant Science macromolecular substances lcsh:Plant culture environment and public health Laboratorium voor Virologie 03 medical and health sciences Arabidopsis transcription factors Transcription factors lcsh:SB1-1110 Transcription factor Original Research Genetics chemistry.chemical_classification biology SUMO conjugation Chemistry Yeast two-hybrid biology.organism_classification In vitro Yeast Cell biology Ubiquitin ligase enzymes and coenzymes (carbohydrates) 030104 developmental biology Enzyme SUMO biology.protein health occupations EPS TCP |
| Zdroj: | Frontiers in Plant Science, 8:2043. Frontiers Media S.A. Frontiers in Plant Science, Vol 8 (2017) Frontiers in Plant Science Frontiers in Plant Science, 8 Frontiers in Plant Science 8 (2017) |
| ISSN: | 1664-462X |
| Popis: | In Arabidopsis more than 400 proteins have been identified as SUMO targets, both in vivo and in vitro. Among others, transcription factors (TFs) are common targets for SUMO conjugation. Here we aimed to exhaustively screen for TFs that interact with the SUMO machinery using an arrayed yeast two-hybrid library containing more than 1,100 TFs. We identified 76 interactors that foremost interact with the SUMO conjugation enzyme SCE1 and/or the SUMO E3 ligase SIZ1. These interactors belong to various TF families, which control a wide range of processes in plant development and stress signaling. Amongst these interactors, the TCP family was overrepresented with several TCPs interacting with different proteins of the SUMO conjugation cycle. For a subset of these TCPs we confirmed that the catalytic site of SCE1 is essential for this interaction. In agreement, TCP1, TCP3, TCP8, TCP14, and TCP15 were readily SUMO modified in an E. coli sumoylation assay. Strikingly, these TCP-SCE1 interactions were found to redistribute these TCPs into nuclear foci/speckles, suggesting that these TCP foci represent sites for SUMO (conjugation) activity. |
| Databáze: | OpenAIRE |
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