Preparation and in vitro/in vivo evaluations of dimpled poly(l-lactic acid) fibers mixed/coated with hydroxyapatite nanocrystals
| Autor: | Kazuo Takakuda, Masahiro Okada, Hiroshi Yanagida, Shigeyuki Nakano, Tsutomu Furuzono, Miwa Masuda, Isao Narama, Satoshi Kitao |
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| Rok vydání: | 2011 |
| Předmět: |
Male
Materials science Nonwoven fabric Polymers Polyesters Biomedical Engineering Medicine (miscellaneous) Nanoparticle engineering.material Rats Sprague-Dawley Biomaterials stomatognathic system Coating Absorbable Implants parasitic diseases Cell Adhesion Animals Lactic Acid Fiber Osteoblasts Nanocomposite Skull technology industry and agriculture Electrospinning Rats Polyester Durapatite Nanocrystal Chemical engineering engineering Nanoparticles Cardiology and Cardiovascular Medicine |
| Zdroj: | Journal of Artificial Organs. 14:331-341 |
| ISSN: | 1619-0904 1434-7229 |
| Popis: | A novel hydroxyapatite (HAp)/poly(L-lactic acid) (PLLA) nanocomposite nonwoven fabric, which was coated and mixed with calcined HAp nanocrystals, and has submicron-sized dimples on its surface, was fabricated. First, HAp-mixed PLLA fabric was prepared by electrospinning a HAp nanocrystal dispersion in dichloromethane (DCM)-dissolved PLLA. It was found that most of the HAp nanocrystals were not exposed on the HAp-mixed PLLA fiber surface but covered with the PLLA matrix. A HAp-nanocrystal coating was applied onto the surface of the HAp-mixed PLLA fabric after corona discharge treatment followed by ethanol washing. The submicron-sized dimples were enlarged after the ethanol washing. After the HAp-nanocrystal coating, the HAp-mixed PLLA fabric surface was uniformly coated with the HAp nanocrystals. In vitro cell spread tests showed that the rat osteoblasts spread more on HAp-nanocrystal-coated fabrics than on non-HAp-coated fabrics. Upon covering calvarial defects, the in vivo hard tissue responses suggested earlier restoration of the defects with HAp-nanocrystal-coated fabrics than those with non-HAp-coated fabrics. |
| Databáze: | OpenAIRE |
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