Spatial Organization of Functional Groups on Bioactive Supramolecular Glycopeptide Nanofibers for Differentiation of Mesenchymal Stem Cells (MSCs) to Brown Adipogenesis
| Autor: | Ayse B. Tekinay, Melis Sardan Ekiz, Ozum S. Caliskan, Mustafa O. Guler |
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| Přispěvatelé: | Güler, Mustafa O. |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cell viability Nanofibers Pharmaceutical Science Mesenchymal stroma cell Biocompatible Materials Carboxylic acid Tissue engineering Surfactant Cell differentiation Cell proliferation Cells Cultured Mesenchymal stem cell Adipogenesis Mesenchymal Stromal Cells Tissue Scaffolds Chemistry Glycopeptides Tissue scaffold Glycopeptide Peptide synthesis Adipocytes Brown Biochemistry Ultrastructure Glutamic acid Amphophile Fat droplet Biotechnology Biomedical Engineering Bioengineering Article Brown adipocyte Marker gene Cell Line Surface-Active Agents 03 medical and health sciences Cell Adhesion Peptide amphiphile Animals Obesity Cell adhesion Amine Cell Proliferation Pharmacology Tissue Engineering Animal Organic Chemistry Spatial analysis Mesenchymal Stem Cells Nanofiber Biomaterial Rats Glucose Metabolism 030104 developmental biology Cell culture Functional group Rat Gene expression Supramolecular chemistry Cytology |
| Zdroj: | Bioconjugate Chemistry |
| ISSN: | 1520-4812 1043-1802 |
| DOI: | 10.1021/acs.bioconjchem.6b00632 |
| Popis: | Spatial organization of bioactive moieties in biological materials has significant impact on the function and efficiency of these systems. Here, we demonstrate the effect of spatial organization of functional groups including carboxylate, amine, and glucose functionalities by using self-assembled peptide amphiphile (PA) nanofibers as a bioactive scaffold. We show that presentation of bioactive groups on glycopeptide nanofibers affects mesenchymal stem cells (MSCs) in a distinct manner by means of adhesion, proliferation, and differentiation. Strikingly, when the glutamic acid is present in the glycopeptide backbone, the PA nanofibers specifically induced differentiation of MSCs into brown adipocytes in the absence of any differentiation medium as shown by lipid droplet accumulation and adipogenic gene marker expression analyses. This effect was not evident in the other glycopeptide nanofibers, which displayed the same functional groups but with different spatial organization. Brown adipocytes are attractive targets for obesity treatment and are found in trace amounts in adults, which also makes this specific glycopeptide nanofiber system an attractive tool to study molecular pathways of brown adipocyte formation. |
| Databáze: | OpenAIRE |
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