Genome-wide mapping of Vibrio cholerae VpsT binding identifies a mechanism for c-di-GMP homeostasis
| Autor: | Thomas Guest, David C. Grainger, James R. J. Haycocks, Gemma Z. L. Warren |
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| Rok vydání: | 2021 |
| Předmět: |
Operon
Diguanylate cyclase activity AcademicSubjects/SCI00010 Regulatory Sequences Nucleic Acid medicine.disease_cause chemistry.chemical_compound Bacterial Proteins Guanosine monophosphate medicine Genetics Homeostasis Binding site Transcription factor Cyclic GMP Vibrio cholerae Mutation biology Chemistry Escherichia coli Proteins Gene regulation Chromatin and Epigenetics Cell biology biology.protein Diguanylate cyclase Phosphorus-Oxygen Lyases Protein Binding Transcription Factors |
| Zdroj: | Nucleic Acids Research |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkab1194 |
| Popis: | Many bacteria use cyclic dimeric guanosine monophosphate (c-di-GMP) to control changes in lifestyle. The molecule, synthesised by proteins having diguanylate cyclase activity, is often a signal to transition from motile to sedentary behaviour. In Vibrio cholerae, c-di-GMP can exert its effects via the transcription factors VpsT and VpsR. Together, these proteins activate genes needed for V. cholerae to form biofilms. In this work, we have mapped the genome-wide distribution of VpsT in a search for further regulatory roles. We show that VpsT binds 23 loci and recognises a degenerate DNA palindrome having the consensus 5’-W-5R-4[CG]-3Y-2W-1W+1R+2[GC]+3Y+4W+5-3’. Most genes targeted by VpsT encode functions related to motility, biofilm formation, or c-di-GMP metabolism. Most notably, VpsT activates expression of the vpvABC operon that encodes a diguanylate cyclase. This creates a positive feedback loop needed to maintain intracellular levels of c-di-GMP. Mutation of the key VpsT binding site, upstream of vpvABC, severs the loop and c-di-GMP levels fall accordingly. Hence, as well as relaying the c-di-GMP signal, VpsT impacts c-di-GMP homeostasis. |
| Databáze: | OpenAIRE |
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