Perturbation of the ubiquitin system causes leaf curling, vascular tissue alterations and necrotic lesions in a higher plant
| Autor: | Jozef Schell, Frank Becker, Andreas Bachmair, R. V. Masterson |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Protein Denaturation
Necrosis Arginine Nicotiana tabacum Proteolysis Molecular Sequence Data Gene Expression Sensitivity and Specificity General Biochemistry Genetics and Molecular Biology Ubiquitin Gene expression Tobacco medicine RNA Messenger Molecular Biology Ubiquitins Vascular tissue Plant Diseases Genetics General Immunology and Microbiology biology medicine.diagnostic_test Base Sequence General Neuroscience Lysine fungi food and beverages Genetic Variation biology.organism_classification Cell biology Plants Toxic biology.protein medicine.symptom Research Article |
| Popis: | A ubiquitin variant with Lys48 changed to Arg acts in vitro as an inhibitor of ubiquitin dependent protein degradation. To assess the role of this proteolytic pathway in the life cycle of plants, we expressed the ubiquitin variant in Nicotiana tabacum. Expression of variant mono- or polyubiquitin leads to marked abnormalities in vascular tissue. In addition, overexpression of variant polyubiquitin induces discrete lesions on leaves. This indicates that perturbations of the ubiquitin system can induce a programmed necrotic response in plants. |
| Databáze: | OpenAIRE |
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