Characterization of Echinococcus granulosus of Spanish origin by DNA restriction endonuclease analysis and Southern blot hybridization
| Autor: | Donald P. McManus, C. Cuesta-Bandera, A.K. Rishi |
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| Rok vydání: | 1988 |
| Předmět: |
Biology
chemistry.chemical_compound Endonuclease parasitic diseases Animals Horses Echinococcus granulosus Southern blot Genetics Sheep Hybridization probe Nucleic Acid Hybridization DNA DNA Restriction Enzymes biology.organism_classification Molecular biology Echinococcus Restriction enzyme Infectious Diseases chemistry Parasitology Spain biology.protein Cattle Restriction fragment length polymorphism |
| Zdroj: | International Journal for Parasitology. 18:137-141 |
| ISSN: | 0020-7519 |
| DOI: | 10.1016/0020-7519(88)90050-1 |
| Popis: | Cuesta-Bandera C., Mcmanus D. P. and Rishi A. K. 1988. Characterization of Echinococcus granulosus of Spanish origin by DNA restriction endonuclease analysis and Southern blot hybridization. International Journal for Parasitology 18 : 137–141. Cloned DNA segments have been used as DNA probes in restriction endonuclease analysis and Southern blot hybridization to characterize E. granulosus material from Spain. Two genetic variants-a sheep/cattle/human form, very similar to the United Kingdom sheep strain, and a parasite from donkeys, genetically similar to the United Kingdom horse strain—have been identified using this approach. It is likely that these two variants represent distinct strains of E. granulosus although further assessment of the biological characteristics of both forms is now required to substantiate the DNA analysis. |
| Databáze: | OpenAIRE |
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