Three-stage continuous culture system with a self-generated anaerobia to study the regionalized metabolism of the human gut microbiota
| Autor: | Pierre Peyret, Monique Alric, Jean-Louis Sébédio, Jean-François Brugère, Nadia Gaci, David Feria-Gervasio, Jean-Michel Cardot, Jean-François Martin, Estelle Pujos, William Tottey |
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| Přispěvatelé: | Conception, Ingénierie et Développement de l'Aliment et du Médicament (CIDAM), Université d'Auvergne - Clermont-Ferrand I (UdA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, EA CIDAM 448, Université d'Auvergne - Clermont-Ferrand I (UdA)-Faculté de Médecine et de Pharmacie, European Union (UE), Auvergne Council, Auvergne Council |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Volunteers Microbiology (medical) Microarray Process improvement Gut microbiota Computational biology Biology Gut flora Microbiology Mass Spectrometry 03 medical and health sciences chemistry.chemical_compound Bioreactors Human gut Humans Anaerobiosis Intestine Large Molecular Biology 030304 developmental biology Fermentation gas human intestinal bacteria 0303 health sciences Three stage 030306 microbiology Microbiota In-vitro simulation of the colon Metabolism Models Theoretical Microarray Analysis biology.organism_classification Biota chemistry Female Distal colon Xenobiotic 3S-ECSIM [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Chromatography Liquid HuGChip |
| Zdroj: | Journal of Microbiological Methods Journal of Microbiological Methods, Elsevier, 2014, 96, pp.111-118. ⟨10.1016/j.mimet.2013.11.015⟩ Journal of Microbiological Methods, 2014, 96, pp.111-118. ⟨10.1016/j.mimet.2013.11.015⟩ |
| ISSN: | 0167-7012 1872-8359 |
| Popis: | This work was supported by a postdoctoral scholarship support from the European Union (UE) and the Auvergne Council, to D. F.-G. (FEDER), by a PhD Scholarship support from the European Union (UE) and the Auvergne Council to W.T. (FEDER), and to a PhD Scholarship support from the French "Ministere de l'Enseignement Superieur et de la Recherche". We thank Pascal Vandekerckove from Lesaffre SA and Zsolt Popse from Global Process Concept (France) for their support. J.-F.B. thanks Celine Vidal and Claire Ardaens for their technical help.; The technical and ethical difficulties in studying the gut microbiota in vivo warrant the development and improvement of in vitro systems able to simulate and control the physicochemical factors of the gut biology. Moreover, the functional regionalization of this organ implies a model simulating these differences. Here we propose an improved and alternative three-stage continuous bioreactor called 3S-ECSIM (three-stage Environmental Control System for Intestinal Microbiota) to study the human large intestine. Its main feature compared with other in vitro systems is the anaerobic atmosphere originating directly from the microbiota metabolism, leading to different gas ratios of CO2 and H-2 in each compartment Analyses of the metabolic and microbiological profiles (LC-MS and a phylogenetic microarray) show different profiles together with a maintenance of this differentiation between the three compartments, simulating respectively a proximal, a transversal and a distal colon. Moreover, the last reactor presents a high similarity with the initial fecal sample, at the microbiological diversity level. Based on our results, this in-vitro process improvement is a valuable alternative tool to dynamically study the structure and metabolism of gut microbiota, and its response to nutrients, prebiotics, probiotics, drugs or xenobiotics. (C) 2013 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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