Cryopreservation of hematopoietic cells using a pre-constituted, protein-free cryopreservative solution with 5% dimethyl sulfoxide
| Autor: | Jane Reese Koc, R. M. Fox, Marcos de Lima, Folashade Otegbeye, Tabatha Trummer |
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| Rok vydání: | 2020 |
| Předmět: |
Adult
Blood Platelets Male Cancer Research Time Factors Platelet Engraftment Cell Survival Neutrophils Immunology CD34 Antigens CD34 Cryopreservation Andrology chemistry.chemical_compound Cryoprotective Agents Immunology and Allergy Humans Platelet Dimethyl Sulfoxide Progenitor cell Genetics (clinical) Aged Transplantation Neutrophil Engraftment Chemistry Dimethyl sulfoxide Hematopoietic Stem Cell Transplantation Cell Biology Middle Aged Hematopoietic Stem Cells Oncology Female |
| Zdroj: | Cytotherapy. 22(11) |
| ISSN: | 1477-2566 |
| Popis: | Background aims Adequate cryopreservation techniques are critical to ensure optimal recovery of functional progenitor cells in hematopoietic cell (HC) transplantation, minimize risk of contamination and prevent infusion-related adverse events (irAEs). In this article, we provide graft function and infusion safety results observed by decreasing the concentration of dimethyl sulfoxide (DMSO) in cryopreservative media and by minimizing processor-dependent formulation. Methods Ten HC products, collected after standard mobilization of multiple myeloma patients, were cryopreserved with PRIME-XV FreezIS (FreezIS) and compared with products previously cryopreserved with media formulated in-house to achieve a final DMSO concentration of 10% (Std10) and 5% (Std5). At infusion, HCs were analyzed for recovery of CD34+ cells and viability; irAEs and time to engraftment of neutrophils and platelets were also monitored. Results Median CD34+ cell recovery for HC cryopreserved with Std10, Std5 and FreezIS was 38%, 78% and 68%, respectively (P = 0.0002). There were less frequent irAEs with Std5 and FreezIS (10%) compared with Std10 (80%) (P ≤ 0.0001). Median time to neutrophil engraftment was comparable (11 days) for all three groups, while platelet engraftment occurred at a median of 20, 19 and 17 days, respectively (p-values not significant). Conclusions FreezIS, a Good Manufacturing Practice-grade, pre-constituted cryopreservative with low DMSO content, maintains functional viability of the HC product while reducing the incidence of irAEs compared with 10% DMSO solutions. The pre-constituted nature of this agent also decreases processor-dependent handling, hence decreasing the risk of variability and infection. |
| Databáze: | OpenAIRE |
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