Utility of quantitative real time PCR in detection and monitoring of viral infections in post renal transplant recipients
| Autor: | Mukut Minz, Shashi Anand, Ranjana W. Minz, H.S. Kohli, Sarabpreet Singh, Yashwant Kumar, Ritambhra Nada, P. Shivanesan, Ashish Sharma, Deepesh B Kanwar |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
medicine.medical_specialty
medicine.medical_treatment Quantitative real-time polymerase chain reaction lcsh:Surgery 030232 urology & nephrology Congenital cytomegalovirus infection Cytomegalovirus 030230 surgery medicine.disease_cause law.invention Epstein–Barr virus 03 medical and health sciences 0302 clinical medicine law Internal medicine medicine Risk factor Polymerase chain reaction Transplantation business.industry Immunosuppression lcsh:RD1-811 medicine.disease Diarrhea Renal transplant Immunology medicine.symptom business |
| Zdroj: | Indian Journal of Transplantation, Vol 10, Iss 1, Pp 9-14 (2016) |
| ISSN: | 2212-0017 |
| Popis: | Background Viral infections cause significant morbidity and mortality in post-transplant period. A highly sensitive and specific detection tool if used may help in early diagnosis and better management in these patients. The study aimed to assess the utility of quantitative real-time polymerase chain reaction (qRT-PCR) as a diagnostic and monitoring tool for viral infections in post renal transplant patients. Methods A quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect EBV and CMV infection in 50 patients on 1st, 2nd, 3rd, and after 6 months of renal transplantation. Results CMV infection was found in 34%, EBV in 28% of recipients, and 17% showed dual infection. Viruses were detectable after the first month of transplantation followed by symptomatic infections within first three months of follow-up, with diarrhea being the commonest symptom. These patients were also at high risk for developing other infections. Anti-thymocyte globulin (ATG) induction was a definitive risk factor for CMV/EBV infection in post operative period. Conclusion Renal transplant patients frequently develop one or more viral infections at a time. Regular monitoring with qRT-PCR and prompt antiviral therapy with reduction in immunosuppression may be an ideal approach for management of these patients. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|