Structural investigation of donor age effect on human bone marrow mesenchymal stem cells: FTIR spectroscopy and imaging
| Autor: | Feride Severcan, Ceren Aksoy, Duygu Uckan, Fatima Aerts Kaya, Bülent Barış Kuşkonmaz |
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| Přispěvatelé: | Çocuk Sağlığı ve Hastalıkları, Kök Hücre Bilimleri |
| Rok vydání: | 2014 |
| Předmět: |
Adult
Male Aging Adolescent Cellular differentiation ATR-FTIR spectroscopy R/W - Tıp Bone Marrow Cells Stem cell aging Article FTIR imaging Bone marrow mesenchymal stem cells Young Adult chemistry.chemical_compound Donor age effect Spectroscopy Fourier Transform Infrared Humans Child Cells Cultured Cell Proliferation Glycogen Cell growth Mesenchymal stem cell Infant Newborn Infant Cell Differentiation Mesenchymal Stem Cells General Medicine Middle Aged Hematopoietic Stem Cells Molecular medicine Haematopoiesis Biochemistry chemistry Child Preschool Multiprotein Complexes Attenuated total reflection Female Geriatrics and Gerontology Stem cell |
| Zdroj: | AGE. 36 |
| ISSN: | 1574-4647 0161-9152 |
| Popis: | Stem cell studies hold enormous potential for development of new therapies for tissue regeneration and repair. Bone marrow mesenchymal stem cells (BM-MSCs) can differentiate into a variety of non-hematopoietic tissues and contribute maintenance of healthy hematopoiesis by providing supportive cellular microenvironment into BM. Here, we investigated age-related differences in BM-MSCs by using attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy and FTIR imaging together with hierarchical clustering as a novel methods to clarify global alterations in the structure and function of macromolecules in characterized BM-MSCs of different aged donors. The results may contribute to identification of age-related new molecular marker(s) to determine the effects of donor age on MSCs. The spectral results reflected that there were significant increases in the concentration of saturated lipids, proteins, glycogen, and nucleic acids in children and adolescent group BM-MSCs when compared to the infants and early and mid adults. The concentration of mentioned macromolecules in adult (early and mid) BM-MSCs were significantly lower than the concentrations in the children and adolescents. These results were attributed to the increase in the proliferation activity in younger BM-MSCs. The distribution of macromolecules into the cells was shown as in the form of chemical maps by FTIR imaging, and the results are in agreement with the ATR-FTIR spectroscopy results. The cellular activity degree was determined by the thiazolyl blue tetrazolium bromide (MTT) proliferation assay to support ATR-FTIR spectroscopy results. BM-MSCs of five different age groups were discriminated by making the hierarchical cluster analysis where the spectral data according to alterations in structure and composition of macromolecules were considered. |
| Databáze: | OpenAIRE |
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