Evaluation of the biological behaviour of various dental implant abutment materials on attachment and viability of human gingival fibroblasts
| Autor: | Panagiotis Pandoleon, Athina Bakopoulou, Petros Koidis, Lambrini Papadopoulou |
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| Rok vydání: | 2018 |
| Předmět: |
Ceramics
Materials science Scanning electron microscope Surface Properties medicine.medical_treatment Cell 02 engineering and technology law.invention 03 medical and health sciences Dental Materials 0302 clinical medicine Confocal microscopy law Biological property Materials Testing medicine Humans General Materials Science MTT assay Yttrium Dental implant General Dentistry Dental Implants Titanium 030206 dentistry Fibroblasts 021001 nanoscience & nanotechnology medicine.anatomical_structure Mechanics of Materials Microscopy Electron Scanning Zirconium 0210 nano-technology Abutment (dentistry) Implant abutment Biomedical engineering |
| Zdroj: | Dental materials : official publication of the Academy of Dental Materials. 35(7) |
| ISSN: | 1879-0097 |
| Popis: | Objective This study aimed to investigate the biological effects of yttria-stabilized zirconia (Y-TZP) compared to other dental implant abutment materials, i.e. lithium disilicate (LS2) and titanium alloy (Ti), as well as the effects of aging of Y-TZP on viability/proliferation and attachment properties of Human Gingival Fibroblasts (HGFs). Methods Cylindrical specimens of each material were prepared as per manufacturer’s instructions. Y-TZP specimens were divided into three groups: 1. no aging (Zr0), 2. aging for 5 h, 134 °C, 2 bars, 100% humidity (Zr5), 3. aging for 10 h under the same conditions (Zr10). Surface roughness was evaluated by optical profilometry; cell metabolic activity/viability by MTT assay, morphological changes by Scanning Electron Microscopy (SEM) and ratio of live/dead cells by confocal microscopy. Results Results showed statistically significant reduction of HGF metabolic activity/viability in contact with Y-TZP after aging. Nevertheless, non-aged zirconia showed no significant differences compared with LS2, Ti and control cultures. In contrast, significant stimulation of cell metabolic activity/viability was observed in HGFs exposed to LS2 eluates. Differential morphological patterns were observed for HGF in contact with different materials/treatments, with obviously increased number of dead cells and sparser distribution of HGFs cultured on Zr10 specimens. These effects were not correlated with surface topography, since Y-TZP aging did not alter surface micro-roughness. Significance These findings indicate that Y-TZP shows comparable biological properties to Ti and LS2 as implant abutment material. Nevertheless, Y-TZP aging might influence gingival cell attachment and proliferation properties, providing an alert to a potentially negative effect on the long-term maintenance of gingival architecture. |
| Databáze: | OpenAIRE |
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