LC-mass spectrometry analysis of N- and C-terminal boundary sequences of polypeptide fragments by limited proteolysis
| Autor: | Justin G. Stroh, Pat Loulakis, Anthony J. Lanzetti, Julie Xie |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
chemistry.chemical_classification
Spectrometry Mass Electrospray Ionization medicine.diagnostic_test Proteolysis Activin Receptors Molecular Sequence Data Thermolysin Peptide Mass spectrometry Proteomics Cleavage (embryo) Peptide Mapping Protein tertiary structure Peptide Fragments Amino acid Protein Structure Tertiary Molecular Weight Protein structure Biochemistry chemistry Structural Biology medicine Amino Acid Sequence Spectroscopy Chromatography High Pressure Liquid |
| Zdroj: | Journal of the American Society for Mass Spectrometry. 16(1) |
| ISSN: | 1044-0305 |
| Popis: | Limited proteolysis is an important and widely used method for analyzing the tertiary structure and determining the domain boundaries of proteins. Here we describe a novel method for determining the N- and C-terminal boundary amino acid sequences of products derived from limited proteolysis using semi-specific and/or non-specific enzymes, with mass spectrometry as the only analytical tool. The core of this method is founded on the recognition that cleavage of proteins with non-specific proteases is not random, but patterned. Based on this recognition, we have the ability to determine the sequence of each proteolytic fragment by extracting a common association between data sets containing multiple potential sequences derived from two or more different mass spectral molecular weight measurements. Proteolytic product sequences derived from specific and non-specific enzymes can be accurately determined without resorting to the conventional time-consuming and laborious methods of SDS-PAGE and N-terminal sequencing analysis. Because of the sensitivity of mass spectrometry, multiple transient proteolysis intermediates can also be identified and analyzed by this method, which allows the ability to monitor the progression of proteolysis and thereby gain insight into protein structures. |
| Databáze: | OpenAIRE |
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