Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family
| Autor: | Xiaoyong Lu, Kangjie Chen, Xiuyan Yu, Cong Luo, Fuming Qiu, Yongliang Zhu, Jian Huang, Qifeng Jing, Jia Li, Linrong Pang, Jiahui Yang, Wo Zhang, Tiejun Jiang |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Programmed cell death
Apoptosis Toxicology chemistry.chemical_compound Alkaloids Matrine Stomach Neoplasms Cell Line Tumor Puma Humans Matrines Cell Proliferation Caspase 7 biology Caspase 3 Cell growth Bcl-2 family biology.organism_classification In vitro Proto-Oncogene Proteins c-bcl-2 chemistry Cell culture Immunology Cancer research Quinolizines |
| Zdroj: | Toxicology. 229:245-252 |
| ISSN: | 0300-483X |
| DOI: | 10.1016/j.tox.2006.10.020 |
| Popis: | Matrine, one of the main active components from the dry roots of Sophora flavescence, was known to induce apoptosis in a variety of tumor cells in vitro. However, the molecular mechanism of cell apoptosis induced by Matrine remains elusive. Here, we investigated the apoptosis in Matrine-treated human gastric cancer MKN45 cells. The results showed that Matrine could inhibit cell proliferation and induce apoptosis in a dose-dependent manner. Further immunoblots revealed that in Matrine-treated cells, caspase-3, -7 were activated and the pro-apoptotic molecules Bok, Bak, Bax, Puma, and Bim were also up-regulated. Our results suggested that Matrine induced gastric cancer MKN45 cells apoptosis via increasing pro-apoptotic molecules of Bcl-2 family. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect