Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancer
| Autor: | Sandip Chavan, Remya Raja, Samapika Routray, Rekha V. Kumar, Sushmita Ghosal, Neeta Mohanty, Keshava K. Datta, Aditi Chatterjee, Akhilesh Pandey, Vishalakshi Nanjappa, Arnab Pal, Rafael Guerrero-Preston, Premendu P. Mathur, Chaluvarayaswamy Ramesha, Ankit P. Jain, Vijay C. Raghu, Aneesha Radhakrishnan, Don Mathew, Reetu Thakur, Ritesh Jadav, Joseph A. Califano, Harsha Gowda, Vinuth N Puttamallesh, Arun H. Patil, Hitendra S. Solanki, KB Linge Gowda, Anu Jain, T. S. Keshava Prasad, Santosh Renuse, Jay Gopal Ray, Mandakulutur S Ganesh, Bipin G. Nair, David Sidransky |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Saliva business.industry Head and neck cancer lcsh:Surgery Cancer lcsh:RD1-811 Protein tyrosine phosphatase Receptor type lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.disease lcsh:RC254-282 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Smokeless tobacco Tobacco users 030220 oncology & carcinogenesis Cancer research medicine PTPRS business |
| Zdroj: | Translational Research in Oral Oncology, Vol 3 (2018) |
| ISSN: | 2057-178X |
| DOI: | 10.1177/2057178x18800534 |
| Popis: | Objectives: To identify potential candidate proteins which are secretory in nature and present at a higher abundance in oral cancer patients with tobacco habits. Methods: Conditioned media of tobacco-treated and -untreated non-neoplastic oral keratinocytes were analyzed using iTRAQ-based mass spectrometry. Hypersecreted proteins; SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1 (SPOCK1); prosaposin (PSAP); and protein tyrosine phosphatase, receptor type S (PTPRS) were validated by enzyme-linked immunosorbent assay (ELISA) using saliva samples from oral cancer patients who are tobacco users. Results: Proteomic analysis of tobacco-treated and -untreated cells led to the identification of 2873 proteins. Among these, 378 proteins showed high abundance and 253 proteins showed low abundance (2-fold cutoff) in conditioned-media of tobacco-treated cells. ELISA-based validation showed significantly higher levels of SPOCK1, PSAP, and PTPRS in oral cancer patients with tobacco chewing habits compared to healthy controls. However, PSAP showed low specificity compared to SPOCK1 and PTPRS. Conclusions: This study indicates significantly increased levels of SPOCK1, PSAP, and PTPRS in saliva of oral cancer patients with tobacco habits. These protein biomarkers might be useful to identify tobacco users with high risk of developing oral cancers. |
| Databáze: | OpenAIRE |
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