Conjugated linoleic acid, unlike other unsaturated fatty acids, strongly induces glutathione synthesis without any lipoperoxidation
| Autor: | Adrien Rossary, Khelifa Arab, Laurent Soulère, Jean-Paul Steghens |
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| Přispěvatelé: | Prot, Josiane, Institut de Chimie et Biochimie Moléculaires et Supramoléculaires (ICBMS), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-École Supérieure Chimie Physique Électronique de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2006 |
| Předmět: |
Models
Molecular Antioxidant Conjugated linoleic acid medicine.medical_treatment Glutathione reductase Medicine (miscellaneous) Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Malondialdehyde medicine Humans Linoleic Acids Conjugated RNA Messenger ComputingMilieux_MISCELLANEOUS Cells Cultured Unsaturated fatty acid 030304 developmental biology 0303 health sciences Arachidonic Acid Nutrition and Dietetics [CHIM.ORGA]Chemical Sciences/Organic chemistry Fatty Acids food and beverages Glutathione Fibroblasts [CHIM.ORGA] Chemical Sciences/Organic chemistry Flow Cytometry Culture Media Oxidative Stress chemistry Biochemistry Cyclooxygenase 2 030220 oncology & carcinogenesis lipids (amino acids peptides and proteins) Arachidonic acid Lipid Peroxidation Reactive Oxygen Species Heme Oxygenase-1 |
| Zdroj: | British Journal of Nutrition British Journal of Nutrition, Cambridge University Press (CUP), 2006, 96 (96), pp.811-819 |
| ISSN: | 1475-2662 0007-1145 |
| DOI: | 10.1017/bjn20061910 |
| Popis: | Enhancementoftheredoxstatusofcellsisacytoprotectivestrategyagainstoxidativedamage.WerecentlyshowedthatDHAupregulatesglutathione(GSH) content via an induction of its related enzymes g-glutamylcysteine ligase and glutathione reductase. In the present study, we investigated theeffectsofeightotherfattyacidsontheredoxstatusandlipidperoxidationofhumanfibroblasts.After48h,onlyarachidonicacidandconjugatedlinoleicacid (CLA) enhanced GSH content through an induction of g-glutamylcysteine ligase. CLA was more potent than arachidonic acid in inducing GSHsynthesis.Forallthefattyacidstested,lipoperoxidation,estimatedbycellmalondialdehydemeasurement,didnotdifferfromthatofcontrolsat48hbutdramatically increased at 7d, except for CLA. Lipoperoxidation is associated at 7d with a high level of reactive oxygen species and with increasedhaemoxygenase-1 and cyclooxygenase-2 mRNA expression. As demonstrated by a tert-butylhydroperoxide cytotoxicity test, the GSH synthesisobtained with arachidonic acid is not sufficient to protect the cells, whereas this protective effect was obvious with CLA at 48h as well as at 7d.The presentresultsshow thatCLAistheonly PUFAable toinduceGSHsynthesis withoutany change inoxidative balance,whereas anupregulationof cyclooxygenase-2 by other PUFA is concomitant with an overproduction of malondialdehyde and reactive oxygen species. The particular hairpinconformation obtained for CLA by molecular modelling could account for this specific biological effect.PUFA: Conjugated linoleic acid: Redox status: Antioxidant response: Glutathione: Cyclooxygenase-2: Haemoxygenase-1: Reactive oxygenspecies: Lipid peroxidation |
| Databáze: | OpenAIRE |
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