Effects of cryopreservation on germinability of olive (Olea europaea L.) pollen
| Autor: | A. De Stradis, V. Bisignano, E. Alba, G. B. Polignano, Vittorio Alba |
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| Rok vydání: | 2011 |
| Předmět: |
Cryopreservation
Germplasm Olea europaea L pollen grain size food and beverages Plant physiology Olive pollen Plant Science Biology medicine.disease_cause biology.organism_classification Agronomy Germination Olea in vitro pollen germinability Pollen otorhinolaryngologic diseases Genetics medicine Cultivar Agronomy and Crop Science Ecology Evolution Behavior and Systematics |
| Zdroj: | Genetic resources and crop evolution 58 (2011): 977–982. doi:10.1007/s10722-011-9736-z info:cnr-pdr/source/autori:Alba V(1).; Bisignano V.(2); Alba E.(3); De Stradis A.(4); G.B. Polignano (2)/titolo:Effects of cryopreservation on germinability of olive (olea europea L.) pollen/doi:10.1007%2Fs10722-011-9736-z/rivista:Genetic resources and crop evolution (Print)/anno:2011/pagina_da:977/pagina_a:982/intervallo_pagine:977–982/volume:58 |
| ISSN: | 1573-5109 0925-9864 |
| DOI: | 10.1007/s10722-011-9736-z |
| Popis: | Long term storage of viable pollen is important for bank germplasm constitution to preserve resources that can be used in breeding programs, biotechnologies and genetic engineering. Pollen from 12 olive (Olea europaea L.) cultivars was stored for 1 year in liquid nitrogen at −196°C. The morphology of pollen grains and germination rates on fresh and long term stored pollen were observed. Results on in vitro pollen germinability, both before and after cryopreservation, showed highly significant responses among the 12 cultivars. The relationship between germinability and pollen grain size did not reveal any significant relationships in both treatments. Our findings will contribute to the improvement of olive pollen preservation and lead to find a more efficient method for its long term storage, while retaining its germinability. |
| Databáze: | OpenAIRE |
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