Stress-induced transcription of satellite III repeats
| Autor: | Saadi Khochbin, Alexandra Metz, Jérôme Govin, Claire Vourc'h, Caroline Jolly, Bryan M. Turner, Marc Vigneron |
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| Přispěvatelé: | Souchier, Catherine, Institut d'oncologie/développement Albert Bonniot de Grenoble (INSERM U823), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), Biotechnologie des interactions macromoléculaires (BIM), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Chromatin and Gene Expression Group, Anatomy Department, University of Birmingham Medical School, INSERM U823, équipe 6 (Epigénétique et Signalisation Cellulaire), Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Joseph Fourier - Grenoble 1 (UJF)-CHU Grenoble-EFS-Institut National de la Santé et de la Recherche Médicale (INSERM), INSERM U823, équipe 10 (Stress et Dynamique de l'Organisation du Génome), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF)-Institut National de la Santé et de la Recherche Médicale (INSERM)-EFS-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF) |
| Rok vydání: | 2003 |
| Předmět: |
Transcription factories
MESH: Protein Transport MESH: Trans-Activators MESH: CREB-Binding Protein Response element RNA polymerase II MESH: Stress [SDV.BC]Life Sciences [q-bio]/Cellular Biology 03 medical and health sciences Stress granule MESH: RNA MESH: Cell Nucleus Structures Heat shock [SDV.BC] Life Sciences [q-bio]/Cellular Biology Transcription factor 030304 developmental biology MESH: Histones 0303 health sciences MESH: Humans General transcription factor biology 030302 biochemistry & molecular biology Sciences du Vivant [q-bio]/Biotechnologies MESH: Transcription Factors Cell Biology Molecular biology MESH: DNA Satellite MESH: RNA Polymerase II MESH: Hela Cells Heat shock factor MESH: Heterochromatin MESH: Trans-Activation (Genetics) biology.protein MESH: Chromosomes Human Pair 9 MESH: Nuclear Proteins MESH: Acetylation MESH: DNA-Binding Proteins |
| Zdroj: | Journal of Cell Biology Journal of Cell Biology, 2004, 164 (1), pp.25-33. ⟨10.1083/jcb.200306104⟩ Journal of Cell Biology, Rockefeller University Press, 2004, 164 (1), pp.25-33. ⟨10.1083/jcb.200306104⟩ |
| ISSN: | 1540-8140 0021-9525 |
| DOI: | 10.1083/jcb.200306104 |
| Popis: | International audience; Exposure of mammalian cells to stress induces the activation of heat shock transcription factor 1 (HSF1) and the subsequent transcription of heat shock genes. Activation of the heat shock response also correlates with a rapid relocalization of HSF1 within a few nuclear structures termed nuclear stress granules. These stress-induced structures, which form primarily on the 9q12 region in humans through direct binding of HSF1 to satellite III repeats, do not colocalize with transcription sites of known hsp genes. In this paper, we show that nuclear stress granules correspond to RNA polymerase II transcription factories where satellite III repeats are transcribed into large and stable RNAs that remain associated with the 9q12 region, even throughout mitosis. This work not only reveals the existence of a new major heat-induced transcript in human cells that may play a role in chromatin structure, but also provides evidence for a transcriptional activity within a locus considered so far as heterochromatic and silent. |
| Databáze: | OpenAIRE |
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