High treatment failure rate is better explained by resistance gene detection than by minimum inhibitory concentration in patients with urogenital Chlamydia trachomatis infection

Autor: Yuan-jun Liu, Lili Shao, Cong You, Quanzhong Liu, Yong Jiang, Junya Cao
Rok vydání: 2020
Předmět:
Adult
Male
0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
030106 microbiology
Erythromycin
Chlamydia trachomatis
Minocycline
Microbial Sensitivity Tests
Drug resistance
Azithromycin
Gene mutation
medicine.disease_cause
Gastroenterology
lcsh:Infectious and parasitic diseases
Young Adult
03 medical and health sciences
Minimum inhibitory concentration
0302 clinical medicine
Antibiotics
Internal medicine
Clarithromycin
Drug Resistance
Bacterial
Humans
Medicine
lcsh:RC109-216
Treatment Failure
030212 general & internal medicine
Doxycycline
tet(M)
business.industry
Minimum inhibitory concentrations
23S rRNA
General Medicine
Chlamydia Infections
Middle Aged
Anti-Bacterial Agents
RNA
Ribosomal
23S
Infectious Diseases
Urinary Tract Infections
Female
Genital Diseases
Male
business
Genital Diseases
Female
medicine.drug
Zdroj: International Journal of Infectious Diseases, Vol 96, Iss, Pp 121-127 (2020)
ISSN: 1201-9712
DOI: 10.1016/j.ijid.2020.03.015
Popis: Objective: The aim of this study was to investigate the relationships between treatment outcomes of patients with urogenital Chlamydia trachomatis infections and minimum inhibitory concentrations (MICs) and drug resistance genes. Methods: The clinical data of 92 patients diagnosed with Chlamydia trachomatis (C. trachomatis) infections were collected. Of these patients, 28 received regular treatment with azithromycin and 64 received minocycline. All patients underwent three monthly follow-ups after the completion of treatment. The microdilution method was used for the in vitro susceptibility tests. The acquisition of 23S rRNA mutations and presence of the tet(M) gene were detected by gene amplification and sequencing. Results: The MICs of azithromycin, clarithromycin, erythromycin, tetracycline, doxycycline, and minocycline were comparable for isolates from the treatment failure and treatment success groups. Higher detection rates of 23S rRNA gene mutations and tet(M) were found in the treatment failure group (57.14% and 71.43%, respectively) than in the treatment success group (14.29% and 30.23%, respectively) (p < 0.05). The A2057G, C2452A, and T2611C gene mutations of 23S rRNA were detected in eight clinical isolates from the azithromycin treatment failure group, while the T2611C gene mutation was detected in one clinical strain from the treatment success group. Conclusions: The detection of resistance genes could better explain the high treatment failure rate than the MIC results in patients with urogenital C. trachomatis infections, highlighting the need for genetic antimicrobial resistance testing in infected patients.
Databáze: OpenAIRE
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