Lymphoma cells contribute to the augmentation of plasma sL-selectins in the serum of lymphoma-bearing mice
| Autor: | Caroline Aubé, Simon D. Bélanger, Yves St-Pierre |
|---|---|
| Přispěvatelé: | Institut Armand Frappier ( INRS-IAF ), Institut National de la Recherche Scientifique [Québec] ( INRS ) -Réseau International des Instituts Pasteur ( RIIP ) -Institut Armand Frappier |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Male
lymphocytes Cancer Research lymphocytes Lymphoid leukemia cell lines and animal models Lymphoid leukemia Polymerase Chain Reaction MESH : Lymphoma T-Cell Mice hemic and lymphatic diseases MESH : Female L-Selectin MESH : Polymerase Chain Reaction biology Hematology Flow Cytometry MESH : Enzyme-Linked Immunosorbent Assay BCL10 [ SDV.MHEP.MI ] Life Sciences [q-bio]/Human health and pathology/Infectious diseases Oncology Female MESH : L-Selectin MESH : Mutation Selectin MESH : Flow Cytometry MESH : Male lymphoma and hodgkin disease Enzyme-Linked Immunosorbent Assay MESH : Mice Inbred C57BL Lymphoma T-Cell In vivo Cell Line Tumor MESH : Mice Extracellular medicine Animals MESH : Neoplasm Transplantation Alleles MESH : Cell Line Tumor medicine.disease Molecular biology MESH : Disease Models Animal In vitro Lymphoma Mice Inbred C57BL Membrane glycoproteins Disease Models Animal Immunology Mutation molecular genetics biology.protein MESH : Animals MESH : Alleles Neoplasm Transplantation |
| Zdroj: | Leukemia & lymphoma Leukemia & lymphoma, Taylor & Francis, 2010, 51 (1), pp.125-31. 〈10.3109/10428190903421177〉 |
| ISSN: | 1042-8194 1029-2403 |
| DOI: | 10.3109/10428190903421177〉 |
| Popis: | International audience; Like many integral membrane glycoproteins, the extracellular domain of L-selectin undergoes rapid shedding, which occurs on both resting and activated host leucocytes. Incubating normal or transformed leukocytes with phorbol esters can also artificially induce shedding of L-selectin, providing multiple possibilities for the source of soluble forms of L-selectin found in the serum of patients with hematological malignancies. Here, using genetically engineered L-selectin-deficient mouse models, we have measured the release of soluble circulating forms of L-selectin in the serum of lymphoma-bearing mice. We found that L-selectin-deficient lymphoma cells could not induce an elevation of circulating soluble forms of L-selectin in normal mice, as compared to lymphoma cells expressing L-selectin. Moreover, soluble forms of L-selectin were detected in the serum in mice bearing lymphoma induced by injection of T lymphoma cells expressing L-selectins. Interestingly, we also found that lymphoma cells that are unable to shed L-selectin in vitro following exposure to phorbol ester can generate soluble forms of serum L-selectin in vivo. Taken together, these results indicate that lymphoma cells are the major contributors to levels of soluble forms of L-selectins in lymphoma-bearing mice. |
| Databáze: | OpenAIRE |
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