The Effect of Mesenteric Lymphadenectomy and Kupffer Cell Depletion on Bacterial Translocation
| Autor: | Anders Stenbäck, Monica Lundholm, Cecilia Johnsson, Johan Wallander, Staffan Meurling, Cristinel Cantar |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Short Bowel Syndrome medicine.medical_specialty Pathology Kupffer Cells medicine.medical_treatment Anti-Inflammatory Agents Gadolinium Chromosomal translocation Spleen Gastroenterology Rats Sprague-Dawley Internal medicine Intestine Small medicine Animals Mesenteric lymph nodes Lung business.industry Kupffer cell Bacterial Infections Short bowel syndrome medicine.disease Immunohistochemistry Rats Survival Rate Transplantation medicine.anatomical_structure Rats Inbred Lew Bacterial Translocation Lymph Node Excision Surgery Lymphadenectomy Lymph Nodes business |
| Zdroj: | Journal of Surgical Research. 102:207-214 |
| ISSN: | 0022-4804 |
| DOI: | 10.1006/jsre.2001.6321 |
| Popis: | Background. Infectious complications are associated with high morbidity in patients with short bowel syndrome and after small bowel transplantation. Bacterial translocation from the intestine is probably an essential factor in the genesis of these infections. In a model for bacterial translocation in the rat we examined the consequence of mesenteric lymphadenectomy and the depletion of Kupffer cells. Materials and methods. The effect of mesenteric lymphadenectomy was studied in two different models; in rats where a Thiry-Vella loop had been created from small bowel and in rats that had received a syngeneic small bowel transplant. To study the role of the Kupffer cells, rats with Thiry-Vella loops were treated intravenously with the Kupffer cell inhibitor gadolinium chloride. All animals were sacrificed on Day 3 postoperatively and the bacterial translocation to the mesenteric lymph nodes, liver, spleen, lung, and blood was evaluated. Results. Removal of the mesenteric lymph nodes did not result in any increased bacterial translocation in animals with a Thiry-Vella loop. However, the inactivation of Kupffer cells with gadolinium chloride produced a more severe translocation to the liver, spleen, and lungs. After small bowel transplantation the bacterial translocation to the spleen was increased in animals without mesenteric lymph nodes. Conclusions. In the model of bacterial translocation from a defunctionalized loop of small bowel the inhibition of Kupffer cells will promote the systemic spread of the translocating bacteria. This indicates an important protective function of the Kupffer cells against translocating microbes. |
| Databáze: | OpenAIRE |
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