Hyaluronan expression following middle cerebral artery occlusion in the rat
| Autor: | Ahmed Al Qteishat, John Gaffney, Jerzy Krupinski, Mark Slevin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Pathology
medicine.medical_specialty Angiogenesis Ischemia Motility Hyaluronoglucosaminidase Gene Expression Regulation Enzymologic Extracellular matrix Rats Sprague-Dawley chemistry.chemical_compound Hyaluronidase Hyaluronic acid medicine Animals RNA Messenger cardiovascular diseases Hyaluronic Acid Stroke biology business.industry Reverse Transcriptase Polymerase Chain Reaction General Neuroscience CD44 Infarction Middle Cerebral Artery medicine.disease Extracellular Matrix Rats Disease Models Animal chemistry biology.protein Female business medicine.drug |
| Popis: | Hyaluronic acid, a major component of the brain extracellular matrix, is a regulator of angiogenesis, cell differentiation and migration. We used the rat middle cerebral artery occlusion model to show hyaluronan accumulation in stroke-affected areas. Using reverse transcription-polymerase chain reaction and Western blotting we showed up-regulation of hyaluronidase-1 and 2 between 1 h and 21 days after stroke. Hyaluronidase-1 was up-regulated earlier than hyaluronidase-2. The hyaladherins, receptor for hyaluronan-mediated motility and CD44 were also increased after stroke. Using immunohistochemistry, we showed association of hyaluronidases 1/2 and hyaladherins with neurons in the infarcted and peri-infarcted regions and hyaluronidase-1 with microvessels. Hyaluronan synthesis and degradation in the stroke hemisphere might have an impact on neuronal survival, angiogenesis and general tissue remodelling after stroke. |
| Databáze: | OpenAIRE |
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