Coupling device for desorption of drugs from solid-phase extraction-pipette tips and on-line gas chromatographic analysis
Autor: | M.W.J van Hout, R.A. de Zeeuw, G.J. de Jong |
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Přispěvatelé: | Faculty of Science and Engineering |
Jazyk: | angličtina |
Rok vydání: | 1999 |
Předmět: |
Chromatography
Gas programmed temperature vaporiser Analytical chemistry Standard solution Mass spectrometry Biochemistry ONLINE TRACE ENRICHMENT Analytical Chemistry law.invention law Desorption Flame ionization detector WATER URINE Sample preparation Solid phase extraction benzodiazepines ORGANIC-COMPOUNDS diazepam POLAR POLLUTANTS instrumentation Chromatography DISKS Elution Chemistry interfaces solid-phase extraction-pipette tips-programmed-temperature vaporiser Organic Chemistry Pipette General Medicine MASS-SPECTROMETRY injection methods lidocaine SYSTEMATIC TOXICOLOGICAL ANALYSIS PARTICLE-LOADED MEMBRANES AQUEOUS SAMPLES |
Zdroj: | Journal of Chromatography A, 858(1), 117-122. ELSEVIER SCIENCE BV |
ISSN: | 0021-9673 |
Popis: | Solid-phase extraction-pipette tips were used for micro solid-phase extraction of lidocaine and diazepam. Off-line desorption was done after in-vial collection for reference purposes, whereas with on-line desorption the eluate was directly introduced in the gas chromatograph. With both methods the total eluate (100 mu l) was introduced into the GC system, which was equipped with a programmed-temperature vaporiser (PTV) for large volume injection. For on-line desorption a laboratory-made coupling device was developed to connect the pipette tips with the injector of the PTV. The coupling device was applied successfully since no leakage occurred at the connection of the coupling device and the pipette tip. No significant differences in recovery of lidocaine and diazepam and in presence of impurities were observed between chromatograms obtained with either off-line or on-line desorption. Preliminary experiments with standard solutions showed recoveries of about 75% for a concentration level of 1 mu g/ml. The system seems particularly suitable for high-throughput analysis. (C) 1999 Published by Elsevier Science B.V. All rights reserved. |
Databáze: | OpenAIRE |
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