Bone morphogenetic protein-7 expression is down-regultaed in human clear cell renal carcinoma
| Autor: | Petar Kes, Josip Pasini, Nikolina Bašić-Jukić, Margareta Radic-Antolic, Daniela Bandic-Pavlovic, Zeljko Kastelan, Tvrtko Hudolin, Renata Zadro, Marijana Ćorić |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Time Factors animal structures Croatia medicine.medical_treatment Bone Morphogenetic Protein 7 Down-Regulation Biology Nephrectomy Disease-Free Survival BMP-7 clear cell carcinoma kidney medicine Humans RNA Messenger Carcinoma Renal Cell Aged Aged 80 and over Kidney Messenger RNA Reverse Transcriptase Polymerase Chain Reaction Cancer Middle Aged medicine.disease Molecular biology Immunohistochemistry Reverse transcriptase Kidney Neoplasms Bone morphogenetic protein 7 medicine.anatomical_structure Treatment Outcome Nephrology Clear cell carcinoma embryonic structures Female |
| DOI: | 10.5301/jn.2010.2020 |
| Popis: | BACKGROUND: Recent studies demonstrated that the expression pattern of bone morphogenetic protein-7 (BMP-7) is altered in different tumors. We determined expression of BMP-7 in human clear cell renal carcinoma (CCRC). ----- METHODS: Samples from cancer and corresponding healthy tissue were obtained from 20 patients who underwent nephrectomy for CCRC. Expression of BMP-7 mRNA was determined by reverse transcriptase polymerase chain reaction (RT-PCR), and protein expression was analyzed by immunohistochemistry. ----- RESULTS: RT-PCR showed strong down-regulation of BMP-7 mRNA in cancer tissue. Immunohistochemistry revealed expression of BMP-7 in normal renal tissue, with almost complete loss of BMP-7 expression in malignant cells of 6 patients (30%). After 3 years of follow-up, 5 out of 6 patients with high BMP-7 mRNA expression were alive and disease-free, compared with 9 out of 14 patients with low BMP-7 mRNA expression. ----- CONCLUSIONS: BMP-7 mRNA and protein expression were down-regulated in CCRC. Further prospective studies are needed to characterize the role of BMP-7 in human CCRC. |
| Databáze: | OpenAIRE |
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