Transcriptome analysis provides insights into the delayed sticky disease symptoms in Carica papaya
| Autor: | Silas P. Rodrigues, Antonio Alberto Ribeiro Fernandes, Johana Madroñero, José A. Ventura, Paolla M. V. Abreu, Tathiana Ferreira Sá Antunes, Patricia Machado Bueno Fernandes |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Flowers Plant Science Biology Real-Time Polymerase Chain Reaction Transcriptome 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Gene expression RNA Viruses RNA Messenger Gene Transcription factor Plant Diseases Plant Proteins Genetics Carica Sequence Analysis RNA Gene Expression Profiling fungi Callose Reproducibility of Results General Medicine biology.organism_classification WRKY protein domain Plant Leaves 030104 developmental biology chemistry Host-Pathogen Interactions Salicylic Acid Agronomy and Crop Science Salicylic acid |
| Zdroj: | Plant Cell Reports. 37:967-980 |
| ISSN: | 1432-203X 0721-7714 |
| Popis: | Global gene expression analysis indicates host stress responses, mainly those mediated by SA, associated to the tolerance to sticky disease symptoms at pre-flowering stage in Carica papaya. Carica papaya plants develop the papaya sticky disease (PSD) as a result of the combined infection of papaya meleira virus (PMeV) and papaya meleira virus 2 (PMeV2), or PMeV complex. PSD symptoms appear only after C. papaya flowers. To understand the mechanisms involved in this phenomenon, the global gene expression patterns of PMeV complex-infected C. papaya at pre-and post-flowering stages were assessed by RNA-Seq. The result was 633 and 88 differentially expressed genes at pre- and post-flowering stages, respectively. At pre-flowering stage, genes related to stress and transport were up-regulated while metabolism-related genes were down-regulated. It was observed that induction of several salicylic acid (SA)-activated genes, including PR1, PR2, PR5, WRKY transcription factors, ROS and callose genes, suggesting SA signaling involvement in the delayed symptoms. In fact, pre-flowering C. papaya treated with exogenous SA showed a tendency to decrease the PMeV and PMeV2 loads when compared to control plants. However, pre-flowering C. papaya also accumulated transcripts encoding a NPR1-inhibitor (NPR1-I/NIM1-I) candidate, genes coding for UDP-glucosyltransferases (UGTs) and several genes involved with ethylene pathway, known to be negative regulators of SA signaling. At post-flowering, when PSD symptoms appeared, the down-regulation of PR-1 encoding gene and the induction of BSMT1 and JA metabolism-related genes were observed. Hence, SA signaling likely operates at the pre-flowering stage of PMeV complex-infected C. papaya inhibiting the development of PSD symptoms, but the induction of its negative regulators prevents the full-scale and long-lasting tolerance. |
| Databáze: | OpenAIRE |
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