T4-like Bacteriophages Isolated from Pig Stools Infect Yersinia pseudotuberculosis and Yersinia pestis Using LPS and OmpF as Receptors
| Autor: | Jin Woo Jun, Maria Pajunen, Mikael Skurnik, Mabruka Salem |
|---|---|
| Přispěvatelé: | HUMI - Human Microbiome Research, Research Programs Unit, Department of Bacteriology and Immunology, Medicum, Glycoscience Group, Helsinki One Health (HOH), Mikael Skurnik / Principal Investigator, HUSLAB, Faculty of Medicine, University of Helsinki, Helsinki University Hospital Area |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides pseudotuberculosis Swine Yersinia pestis Gp38 viruses receptor 030106 microbiology lcsh:QR1-502 Porins Myoviridae Genome Viral Y. pseudotuberculosis Yersinia Genome lcsh:Microbiology Host Specificity Article Microbiology Bacteriophage 03 medical and health sciences Feces Caudovirales Bacterial Proteins bacteriophage Virology Yersinia pseudotuberculosis Animals Bacteriophages Phylogeny 11832 Microbiology and virology Base Composition biology lipopolysaccharide biology.organism_classification 3. Good health 030104 developmental biology Infectious Diseases tail fiber Porin Receptors Virus 3111 Biomedicine |
| Zdroj: | Viruses Volume 13 Issue 2 Viruses, Vol 13, Iss 296, p 296 (2021) |
| ISSN: | 1999-4915 |
| DOI: | 10.3390/v13020296 |
| Popis: | The Yersinia bacteriophages fPS-2, fPS-65, and fPS-90, isolated from pig stools, have long contractile tails and elongated heads, and they belong to genus Tequatroviruses in the order Caudovirales. The phages exhibited relatively wide host ranges among Yersinia pseudotuberculosis and related species. One-step growth curve experiments revealed that the phages have latent periods of 50–80 min with burst sizes of 44–65 virions per infected cell. The phage genomes consist of circularly permuted dsDNA of 169,060, 167,058, and 167,132 bp in size, respectively, with a G + C content 35.3%. The number of predicted genes range from 267 to 271. The phage genomes are 84–92% identical to each other and ca 85% identical to phage T4. The phage receptors were identified by whole genome sequencing of spontaneous phage-resistant mutants. The phage-resistant strains had mutations in the ompF, galU, hldD, or hldE genes. OmpF is a porin, and the other genes encode lipopolysaccharide (LPS) biosynthetic enzymes. The ompF, galU, and hldE mutants were successfully complemented in trans with respective wild-type genes. The host recognition was assigned to long tail fiber tip protein Gp38, analogous to that of T-even phages such as Salmonella phage S16, specifically to the distal β-helices connecting loops. |
| Databáze: | OpenAIRE |
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