Radioimmunoassay for Albuterol Using a Monoclonal Antibody: Application for Direct Quantification in Horse Urine
| Autor: | Sylvie Marleau, D. Sondag, M. Bellemare, Huy Ong, Albert Adam, A. Rapaille, Ph. Raymond, N. Beaulieu, J. C. K. Loo, D. Giroux |
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| Rok vydání: | 1990 |
| Předmět: |
medicine.drug_class
Immunology Antibody Affinity Radioimmunoassay Urine Monoclonal antibody Mice Antibody Specificity medicine Animals Albuterol Horses Bovine serum albumin Pharmacology Detection limit Chromatography biology Chemistry Antibodies Monoclonal Reproducibility of Results Horse Immunoglobulin Isotypes Clenbuterol biology.protein Female Antibody medicine.drug |
| Zdroj: | Journal of Immunoassay. 11:329-345 |
| ISSN: | 0197-1522 |
| Popis: | A monoclonal antibody was synthesized in mouse against the O-(3-carboxypropionyl) derivative of albuterol linked to bovine serum albumin. Isotyping of this material revealed the IgG1 class characterized by an affinity constant of 1.03 nM-1 and a density of sites of 0.55 nM. This antibody was found specific as its cross-reactivity to structurally related molecules was less than 1% except for clenbuterol (75%). A radioimmunoassay was set up with culture supernatant (final dilution 1/1000) and [3H] albuterol. The calibration curve was characterized by a maximum binding of 28%, an ED50 of 1.15 pmol per tube, the detection limit was 28.8 fmol/tube and the linearity of the response was up to 39.8 pmol/tube. This RIA method has been used for direct quantitation of albuterol in horse urine without any clean-up or extraction step. |
| Databáze: | OpenAIRE |
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