Identification of key genes and novel immune infiltration-associated biomarkers of sepsis
| Autor: | Meng Wu, Chao Xu, Jinling Ma, Wendan Tian, Jian-bo Xu, Ling Lu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
lcsh:Immunologic diseases. Allergy
key biomarkers 0301 basic medicine Immunology Cell Naive B cell Biology MMP9 Microbiology Sepsis 03 medical and health sciences 0302 clinical medicine Immune system Cell Movement medicine CEBPB Humans Gene Regulatory Networks KEGG Molecular Biology Gene B-Lymphocytes immune infiltration WGCNA CCAAT-Enhancer-Binding Protein-beta Immunity CIBERSORT Computational Biology 030208 emergency & critical care medicine Original Articles T-Lymphocytes Helper-Inducer Cell Biology Prognosis medicine.disease Receptors Complement Killer Cells Natural Gene Ontology 030104 developmental biology Infectious Diseases medicine.anatomical_structure Matrix Metalloproteinase 9 lcsh:RC581-607 Biomarkers |
| Zdroj: | Innate Immunity Innate Immunity, Vol 26 (2020) |
| ISSN: | 1753-4267 1753-4259 |
| DOI: | 10.1177/1753425920966380 |
| Popis: | Sepsis is the major cause of mortality in the intensive care unit. The aim of this study was to identify the key prognostic biomarkers of abnormal expression and immune infiltration in sepsis. In this study, a total of 36 differentially expressed genes were identified to be mainly involved in a number of immune-related Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways. The hub genes ( MMP9 and C3AR1) were significantly related to the prognosis of sepsis patients. The immune infiltration analysis indicated a significant difference in the relative cell content of naive B cells, follicular Th cells, activated NK cells, eosinophils, neutrophils and monocytes between sepsis and normal controls. Weighted gene co-expression network analysis and a de-convolution algorithm that quantifies the cellular composition of immune cells were used to analyse the sepsis expression data from the Gene Expression Omnibus database and to identify modules related to differential immune cells. CEBPB is the key immune-related gene that may be involved in sepsis. Gene set enrichment analysis revealed that CEBPB is involved in the processes of T cell selection, B cell–mediated immunity, NK cell activation and pathways of T cells, B cells and NK cells. Therefore, CEBPB may play a key role in the biological and immunological processes of sepsis. |
| Databáze: | OpenAIRE |
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