Transcutaneous immunization with a 40-kDa outer membrane protein of Porphyromonas gingivalis induces specific antibodies which inhibit coaggregation by P. gingivalis
| Autor: | Yasuko Shibata, Mitsuo Hayakawa, Yoshimitsu Abiko, Masafumi Yamamoto, Satomi Maeba, Jun Namikoshi, Shigeo Otake |
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| Rok vydání: | 2004 |
| Předmět: |
Saliva
Cholera Toxin chemical and pharmacologic phenomena Spleen medicine.disease_cause Administration Cutaneous complex mixtures Microbiology Mice Adjuvants Immunologic medicine Bacteroidaceae Infections Animals Periodontitis Bacteroidaceae Porphyromonas gingivalis Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology Cholera toxin Public Health Environmental and Occupational Health bacterial infections and mycoses biology.organism_classification medicine.disease Chronic periodontitis Antibodies Bacterial Immunoglobulin A Molecular Weight Infectious Diseases medicine.anatomical_structure Immunoglobulin G Immunology Bacterial Vaccines Models Animal biology.protein bacteria Molecular Medicine Antibody Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | Vaccine. 23(19) |
| ISSN: | 0264-410X |
| Popis: | This study seeks to assess the potential of a 40-kDa outer membrane protein of Porphyromonas gingivalis (40k-OMP) as a transcutaneous vaccine against chronic periodontitis. Transcutaneous immunization (TCI) of mice with 40k-OMP alone elicited 40k-OMP-specific IgG antibody (Ab) responses in both serum and saliva. When administered with cholera toxin (CT) as adjuvant, TCI with 40k-OMP not only elevated IgG Abs as noted above, but also induced IgA responses in serum but not in saliva. Salivary IgG from mice given 40k-OMP alone or 40k-OMP plus CT showed higher binding levels to the 40k-OMP than did that of non-immunized mice. Ab-forming cell (AFC) analysis revealed high numbers of 40k-OMP-specific IgG AFCs in the spleen but low numbers in the salivary glands of mice given 40k-OMP alone or 40k-OMP plus CT. Since 40k-OMP-specific IgG inhibited the coaggregation of P. gingivalis vesicles and S. gordonii, TCI with 40k-OMP may be a useful tool in the quest to prevent P. gingivalis infection. |
| Databáze: | OpenAIRE |
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