Ion-Selective Carbon Nanotube Field-Effect Transistors for Monitoring Drug Effects on Nicotinic Acetylcholine Receptor Activation in Live Cells
| Autor: | Seunghun Hong, Jin-Young Jeong, Viet Anh Pham Ba, Yoon-Ji Choi, Youngtak Cho |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Nicotine
Letter Potassium Chromaffin Cells chemistry.chemical_element 02 engineering and technology Receptors Nicotinic lcsh:Chemical technology Biochemistry PC12 Cells Analytical Chemistry 03 medical and health sciences Valinomycin chemistry.chemical_compound valinomycin Animals lcsh:TP1-1185 Electrical and Electronic Engineering nicotinic acetylcholine receptor carbon nanotube field-effect transistor Instrumentation Electrodes Ion channel 030304 developmental biology Acetylcholine receptor 0303 health sciences Nanotubes Carbon 021001 nanoscience & nanotechnology ion-selective membrane Atomic and Molecular Physics and Optics Carbon nanotube field-effect transistor Rats Nicotinic acetylcholine receptor Nicotinic agonist Membrane chemistry Pharmaceutical Preparations ion channel Biophysics 0210 nano-technology |
| Zdroj: | Sensors Sensors, Vol 20, Iss 3680, p 3680 (2020) Sensors (Basel, Switzerland) |
| ISSN: | 1424-8220 |
| DOI: | 10.3390/s20133680 |
| Popis: | We developed ion-selective field-effect transistor (FET) sensors with floating electrodes for the monitoring of the potassium ion release by the stimulation of nicotinic acetylcholine receptors (nAChRs) on PC12 cells. Here, ion-selective valinomycin-polyvinyl chloride (PVC) membranes were coated on the floating electrode-based carbon nanotube (CNT) FETs to build the sensors. The sensors could selectively measure potassium ions with a minimum detection limit of 1 nM. We utilized the sensor for the real-time monitoring of the potassium ion released from a live cell stimulated by nicotine. Notably, this method also allowed us to quantitatively monitor the cell responses by agonists and antagonists of nAChRs. These results suggest that our ion-selective CNT-FET sensor has potential uses in biological and medical researches such as the monitoring of ion-channel activity and the screening of drugs. |
| Databáze: | OpenAIRE |
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