Delivery of xenon-containing echogenic liposomes inhibits early brain injury following subarachnoid hemorrhage
| Autor: | Hyunggun Kim, James C. Grotta, Jaroslaw Aronowski, Melanie R. Moody, Tao Peng, Melvin E. Klegerman, David D. McPherson, Yi Feng Miao, Shaoling Huang |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Xenon Subarachnoid hemorrhage medicine.medical_treatment lcsh:Medicine Neuroprotection Article Random Allocation 03 medical and health sciences 0302 clinical medicine Hematoma Microscopy Electron Transmission medicine.artery medicine Echogenic liposomes Animals Common carotid artery cardiovascular diseases lcsh:Science Saline Ultrasonography Multidisciplinary business.industry Ultrasound lcsh:R Subarachnoid Hemorrhage medicine.disease Rats 3. Good health nervous system diseases Disease Models Animal Drug Liberation Neuroprotective Agents 030104 developmental biology Apoptosis Brain Injuries Anesthesia Liposomes Administration Intravenous lcsh:Q business 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports, Vol 8, Iss 1, Pp 1-12 (2018) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-18914-6 |
| Popis: | Xenon (Xe), a noble gas, has promising neuroprotective properties with no proven adverse side-effects. We evaluated neuroprotective effects of Xe delivered by Xe-containing echogenic liposomes (Xe-ELIP) via ultrasound-controlled cerebral drug release on early brain injury following subarachnoid hemorrhage (SAH). The Xe-ELIP structure was evaluated by ultrasound imaging, electron microscopy and gas chromatography-mass spectroscopy. Animals were randomly divided into five groups: Sham, SAH, SAH treated with Xe-ELIP, empty ELIP, or Xe-saturated saline. Treatments were administrated intravenously in combination with ultrasound application over the common carotid artery to trigger Xe release from circulating Xe-ELIP. Hematoma development was graded by SAH scaling and quantitated by a colorimetric method. Neurological evaluation and motor behavioral tests were conducted for three days following SAH injury. Ultrasound imaging and electron microscopy demonstrated that Xe-ELIP have a unique two-compartment structure, which allows a two-stage Xe release profile. Xe-ELIP treatment effectively reduced bleeding, improved general neurological function, and alleviated motor function damage in association with reduced apoptotic neuronal death and decreased mortality. Xe-ELIP alleviated early SAH brain injury by inhibiting neuronal death and bleeding. This novel approach provides a noninvasive strategy of therapeutic gas delivery for SAH treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|