Bile acid derivatives from a sponge-associated bacterium Psychrobacter sp
| Autor: | Huayue Li, Hye Ja Lee, Pramod B. Shinde, Sang-Ho Choi, Jee H. Jung, Eun Sook Yoo, Jongki Hong, Chong-O. Lee |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.drug_class
Anti-Inflammatory Agents Antineoplastic Agents Fractionation Nitric Oxide Anti-inflammatory Cell Line Bile Acids and Salts Mice Cell Line Tumor Drug Discovery medicine Animals Humans Psychrobacter Cytotoxicity biology Bile acid Molecular Structure Interleukin-6 Macrophages Organic Chemistry Cholic Acids biology.organism_classification Anti-Bacterial Agents Porifera Sponge Biochemistry Models Chemical Cell culture Molecular Medicine Drug Screening Assays Antitumor Bacteria |
| Zdroj: | Archives of pharmacal research. 32(6) |
| ISSN: | 0253-6269 |
| Popis: | In our search for bioactive metabolites from a marine sponge-associated bacterium Psychrobacter sp., a new bile acid derivative (1), which was assumed to be an artifact, were isolated along with six known (2-7) compounds by bioactivity-guided fractionation. Elucidation of the structure of the new compound was done using a combination of NMR ((1)H, (13)C, HMBC, HSQC, and COSY) and MS spectroscopy. Compound 1 exhibited moderate suppressive effects on both NO and IL-6 production at a concentration of 200 microM (87.3 microg/mL) without significant cytotoxicity against cells. Compounds 2-5 and 7 showed selective inhibitory activity against several human pathogenic bacterial strains at the low concentration of 30 microg/well. In a cytotoxicity evaluation, only compound 7 showed mild cytotoxicity against five human solid tumor cell lines (A-549, SK-OV-3, SK-MEL-2, XF-498, and HCT-15) with ED(50) values in the range of 11-14 microg/mL. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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