Functional genetic characterization of salivary gland development in Aedes aegypti
| Autor: | Christy Le, Emily Andrews, Anthony Clemons, David W. Severson, Chilinh Nguyen, Molly Duman-Scheel, Zeinab Annan, Longhua Sun |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
animal structures
Zoology Aedes aegypti Biology Development Marker gene 03 medical and health sciences 0302 clinical medicine stomatognathic system Mosquito Genetics medicine Melanogaster Ecology Evolution Behavior and Systematics 030304 developmental biology Salivary gland 0303 health sciences Gene knockdown Neuroectoderm Research fungi biology.organism_classification 3. Good health Cell biology medicine.anatomical_structure Drosophila melanogaster Genetic marker siRNA Vector 030217 neurology & neurosurgery Developmental Biology CrebA |
| Zdroj: | EvoDevo |
| ISSN: | 2041-9139 |
| Popis: | BackgroundDespite the devastating global impact of mosquito-borne illnesses on human health, very little is known about mosquito developmental biology. In this investigation, functional genetic analysis of embryonic salivary gland development was performed inAedes aegypti, the dengue and yellow fever vector and an emerging model for vector mosquito development. Although embryonic salivary gland development has been well studied inDrosophila melanogaster, little is known about this process in mosquitoes or other arthropods.ResultsMosquitoes possess orthologs of many genes that regulateDrosophila melanogasterembryonic salivary gland development. The expression patterns of a large subset of these genes were assessed duringAe. aegyptidevelopment. These studies identified a set of molecular genetic markers for the developing mosquito salivary gland. Analysis of marker expression allowed for tracking of the progression ofAe. aegyptisalivary gland development in embryos. InDrosophila,the salivary glands develop from placodes located in the ventral neuroectoderm. However, inAe. aegypti,salivary marker genes are not expressed in placode-like patterns in the ventral neuroectoderm. Instead, marker gene expression is detected in salivary gland rudiments adjacent to the proventriculus. These observations highlighted the need for functional genetic characterization of mosquito salivary gland development. An siRNA- mediated knockdown strategy was therefore employed to investigate the role of one of the marker genes,cyclic-AMP response element binding protein A(Aae crebA),duringAe. aegyptisalivary gland development.These experiments revealed thatAae crebAencodes a key transcriptional regulator of the secretory pathway in the developingAe. aegyptisalivary gland.ConclusionsThe results of this investigation indicated that the initiation of salivary gland development inAe. aegyptisignificantly differs from that ofD. melanogaster. Despite these differences, some elements of salivary gland development, including the ability of CrebA to regulate secretory gene expression, are conserved between the two species. These studies underscore the need for further analysis of mosquito developmental genetics and may foster comparative studies of salivary gland development in additional insect species. |
| Databáze: | OpenAIRE |
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