Initial microbial community of the neonatal stomach immediately after birth
| Autor: | Michael Sioda, Sarah Bajorek, Anthony A. Fodor, Leslie A. Parker, James Johnson, Kathryn Winglee, Nan Li, Michael T. Weaver, Josee Gauthier, Dominick J. Lemas, Christian Jobin, Josef Neu, Graciela L. Lorca |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Male Amniotic fluid Physiology Gestational Age Pilot Projects Bacterial Physiological Phenomena Microbiology Cohort Studies 03 medical and health sciences 0302 clinical medicine RNA Ribosomal 16S Lactobacillus iners medicine Humans Microbiome Gastric Lavage biology Lactobacillus crispatus Bacteria Stomach Gastroenterology Infant Newborn Gestational age Biodiversity biology.organism_classification Bacterial Load Gastrointestinal Microbiome 030104 developmental biology Infectious Diseases medicine.anatomical_structure Cross-Sectional Studies Microbial population biology Cohort Research Paper/Report 030211 gastroenterology & hepatology Female |
| Zdroj: | Gut microbes. 10(3) |
| ISSN: | 1949-0984 |
| Popis: | The purpose of this prospective cross-sectional cohort pilot study is to explore the initial microbial community of gastric aspirate fluid as collected immediately after birth and its relationships with mode of delivery and preterm birth. Twenty-nine gastric aspirate samples collected immediately after birth from infants born between 24–40 weeks gestation were analyzed for microbial composition. Total microbial content was low in many samples, with a substantial number sharing taxonomic composition with negative controls. qPCR targeting the 16S rRNA gene showed that infants delivered vaginally had a higher microbial load than infants delivered by C-section. Some pre-term samples showed high relative abundance of genus Ureaplasma, consistent with previous literature that has implicated infections with this taxon as a potential cause of pre-term birth. Vaginally born term infant samples, by contrast, had significantly higher levels of genus Lactobacillus with Lactobacillus crispatus the most dominant species. Microbial evaluation showed that vaginally born term infant gastric aspirate samples had higher levels of lactobacilli than pre-terms. Samples from many infants had low microbial load near the edge of the detection limit. |
| Databáze: | OpenAIRE |
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